Detailed information of TA system TA139327

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31564..31990
• Replicon: plasmid p3RM8082
• Accession: NZ_CP043827
• Organism: Escherichia coli strain RM8082

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: F1745_RS25945
• Protein ID: WP_096937776.1
• Coordinates: 31564..31689 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	31766..31990 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1745_RS25895 (26595)	26595..26876	-	282	WP_000974581.1	hypothetical protein	-
F1745_RS25900 (27354)	27354..27740	-	387	WP_001137413.1	conjugal transfer relaxosome DNA-binding protein TraM	-
F1745_RS25905 (28212)	28212..28664	+	453	WP_000163901.1	transglycosylase SLT domain-containing protein	-
F1745_RS25910 (28771)	28771..28929	+	159	Protein_27	IS3 family transposase	-
F1745_RS25915 (29090)	29090..29470	-	381	WP_238014250.1	hypothetical protein	-
F1745_RS25920 (29647)	29647..29889	-	243	WP_000540593.1	hypothetical protein	-
F1745_RS25925 (29954)	29954..30210	-	257	Protein_30	hypothetical protein	-
F1745_RS25930 (30366)	30366..30668	-	303	WP_063101366.1	hypothetical protein	-
F1745_RS25935 (30692)	30692..30898	-	207	WP_050436701.1	hypothetical protein	-
F1745_RS25940 (30968)	30968..31326	+	359	Protein_33	hypothetical protein	-
F1745_RS25945 (31564)	31564..31689	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
F1745_RS25950 (31631)	31631..31780	-	150	Protein_35	plasmid maintenance protein Mok	-
- (31768)	31768..31833	-	66	NuclAT_1	-	-
- (31768)	31768..31833	-	66	NuclAT_1	-	-
- (31768)	31768..31833	-	66	NuclAT_3	-	-
- (31771)	31771..31834	+	64	NuclAT_2	-	-
- (31771)	31771..31834	+	64	NuclAT_2	-	-
- (31766)	31766..31990	+	225	NuclAT_1	-	-
- (31766)	31766..31990	-	225	NuclAT_1	-	Antitoxin
- (31766)	31766..31990	-	225	NuclAT_1	-	Antitoxin
- (31766)	31766..31990	-	225	NuclAT_1	-	Antitoxin
- (31766)	31766..31990	-	225	NuclAT_1	-	Antitoxin
F1745_RS25955 (31802)	31802..31990	+	189	WP_163426551.1	protein sok	-
F1745_RS25960 (32057)	32057..32398	-	342	Protein_37	transposase	-
F1745_RS25965 (32432)	32432..33966	-	1535	Protein_38	IS66 family transposase	-
F1745_RS25970 (34016)	34016..34363	-	348	WP_000609174.1	IS66 family insertion sequence element accessory protein TnpB	-
F1745_RS25975 (35322)	35322..36878	+	1557	WP_163426483.1	L-lactate permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T139327 WP_096937776.1 NZ_CP043827:c31689-31564 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T139327 NZ_CP058644:c2778356-2777481 [Pseudomonas yamanorum]
ATGTTACCCAGCGTCAATCACCTGCCATTGACCGCGGACCTCGAGGCCCGCCTGGTCGCCGACTTTCCTCAGCACACGCT
GAAGATCGACGGCGGCCGGCAGGCCCTGCGCGAATGCGGCAGCGGCCCGGCCATCGTCTTGCTCCACGGCATCGGCTCGG
GCGCAGCCTCCTGGTTGCAGGTGGCGCAACAATTGGCCCCCGGCGCTCGGGTGATCGCCTGGGATGCTCCAGGCTATGGC
GACTCCACGCCGCTGGAAGACTCGGCTCCGAAGGCTGCGGCCTATGCCCAACGCCTTTTGCAATTGCTCGACGGCCTGCA
CATCGAGCACTGCGTGCTGGTCGGCCATTCCCTGGGCGCCCTGACCGCCGTGGCGTTTGCCCGGCTGTATGCCAACCGGG
TCAGTCGCCTGGTACTGATCAGCCCGGCCCGTGGTTATGGTGATCCGGCCCGCAGCCAGCAACGTCAGGAAGTGCGCACC
AAACGCCTGCGGTCGCTGGAACAGGTAGGCATCCAGGGCAACGCCGAGCAACGCAGCGCCCATATGGTGTCCGCAAAGGC
CTCGCCACAGGCGCTGGCGTGGGTGCGCTGGAACATGGCGCGGCTCAACCCCGACGGTTATCGCCAGGCCATCGAGCTGC
TGTGCGACGACGACCTGCTGCGCCACACCCCGCTGTCGATGCCGTGTGAAGTGCATTGCGGCCAGGACGACGGCATCACC
CCGCCGGAGGGCTGCCTGCCGCTGGCCAAGGCCCTGGGTACGCATTTGAGCCTGATCCCCGACGCCGGGCACGCCAGCCC
CATCGAACAACCGCTGGTGGTTGCAGGCCGCCTGGCCTTTGCCATCAAAGAATCCCTGACAGGTAGAACCCTATGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT139327 NZ_CP043827:c31990-31766 [Escherichia coli]
TCACACGGATTGCCCGTGAACTGGCTGAACGACCAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGATATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin