Detailed information of TA system TA139322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4474982..4475584
• Replicon: chromosome
• Accession: NZ_CP043825
• Organism: Escherichia coli strain RM8082

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: F1745_RS22155
• Protein ID: WP_000897305.1
• Coordinates: 4474982..4475293 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F1745_RS22160
• Protein ID: WP_000356397.1
• Coordinates: 4475294..4475584 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1745_RS22125 (4470012)	4470012..4470797	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
F1745_RS22130 (4470896)	4470896..4471495	+	600	WP_001295269.1	glucose-1-phosphatase	-
F1745_RS22135 (4471489)	4471489..4472361	+	873	WP_000920762.1	virulence factor BrkB family protein	-
F1745_RS22140 (4472358)	4472358..4472795	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
F1745_RS22145 (4472840)	4472840..4473781	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
F1745_RS22150 (4473845)	4473845..4474753	-	909	WP_001162704.1	alpha/beta hydrolase	-
F1745_RS22155 (4474982)	4474982..4475293	+	312	WP_000897305.1	hypothetical protein	Toxin
F1745_RS22160 (4475294)	4475294..4475584	+	291	WP_000356397.1	NadS family protein	Antitoxin
F1745_RS22165 (4476189)	4476189..4476407	+	219	WP_001297075.1	CopG family transcriptional regulator	-
F1745_RS22170 (4476625)	4476625..4476867	+	243	WP_001086388.1	protein YiiF	-
F1745_RS22175 (4477197)	4477197..4478126	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
F1745_RS22180 (4478123)	4478123..4478758	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
F1745_RS22185 (4478755)	4478755..4479657	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T139322 WP_000897305.1 NZ_CP043825:4474982-4475293 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T139322 NZ_CP058639:670611-670862 [Rhodococcus sp. ZPP]
ATGCGAACTATGACCTACTCGGAGTCTCGCGCGCGGTACGCCGAGGTCCTCAACGCCGTCGTCGATGACCGGGAAGAAAT
CGTCATCACGCGGGCGGGTCACGAGCCGGTGGTGATGGTTTCGCTCGACGACTATGAGTCGTTGAAGGAAACGGCATACT
TGCTCAGGAGTCCGGAGAACGCCCGCCGGCTGTTGGCGTCGATCGATCGGTTGGAGTCCGGCGGGGGAACTGTCCGGGAT
CTGGCTGAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT139322 WP_000356397.1 NZ_CP043825:4475294-4475584 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT139322 NZ_CP058639:670859-671119 [Rhodococcus sp. ZPP]
ATGAAACTTGTCTGGGACGAGTCCGCGTGGGAGGACTACGTCTGGTGGCAGGCGCAGGATCGGAAGATCGTCAAGCGGAT
CAACACACTACTCAAGGATGTCGAACGCAACGGGAACGACGGAATCGGCAAGCCCGAGCCCCTCAAGCACGGTTTTCAAG
GCTACTGGTCCCGGCGTATCACCGATGAGCACCGGCTGGTCTATAAAATTGTCGATGATCAGATCCGCATTGCTGCCTGC
CGTTACCACTACGGACACTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7