Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3567696..3568495 Replicon chromosome
Accession NZ_CP043825
Organism Escherichia coli strain RM8082

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag F1745_RS17800 Protein ID WP_000347266.1
Coordinates 3568031..3568495 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag F1745_RS17795 Protein ID WP_001307405.1
Coordinates 3567696..3568031 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1745_RS17780 (3563481) 3563481..3564251 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
F1745_RS17785 (3564267) 3564267..3565601 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
F1745_RS17790 (3565976) 3565976..3567547 + 1572 WP_001273741.1 galactarate dehydratase -
F1745_RS17795 (3567696) 3567696..3568031 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
F1745_RS17800 (3568031) 3568031..3568495 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
F1745_RS17805 (3568550) 3568550..3569359 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
F1745_RS17810 (3569608) 3569608..3570888 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
F1745_RS17815 (3570911) 3570911..3571384 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
F1745_RS17820 (3571395) 3571395..3572174 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
F1745_RS17825 (3572164) 3572164..3573042 + 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
F1745_RS17830 (3573060) 3573060..3573494 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3556708..3568495 11787


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T139314 WP_000347266.1 NZ_CP043825:3568031-3568495 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T139314 NZ_CP058637:3248131-3248433 [Enterobacter roggenkampii]
ATGAAGGAGATTGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAACTTAAAAGATCGACGAGCGAAGACCATTATCGC
TTCCCGCCTCTTTCGGTTGGCAAATGGCTTAGCGGGCGACGTTAAACCCGTGGGAGAAGGTATAAGTGAGCTAAGGATCC
ACTTTGGTCCGGGCTACAGGATCTATTTTAAAGACCAGGGCAATTACATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTGATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT139314 WP_001307405.1 NZ_CP043825:3567696-3568031 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT139314 NZ_CP058637:3248440-3248727 [Enterobacter roggenkampii]
ATGCATAAATTAACACCTTACGATCCCGCCAACGCGTTGGTGGATGACGAGGAAATCGCCGTGTTTATCGCTGATGCACT
AGAAACAGGCGACTCGACATATATCGCTAAAGCCCTTGGTGTTGTCGCGCGAGCCAAAGGCATGTCGAACATTTCACAGC
AAACCGGTTTATCACGTGAGCAGCTTTATCGGTCATTCAGTGATAAAGGAAACCCAACGCTCAAAACCACGCTCGCGGTC
ATGAAAGCATTGGGTTTTGGGCTAACACTCAAACACGCCGGGGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References