Detailed information of TA system TA139293

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 23477..23903
• Replicon: plasmid p1RM10740
• Accession: NZ_CP043824
• Organism: Escherichia coli strain RM10740

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: F1748_RS26005
• Protein ID: WP_001372321.1
• Coordinates: 23477..23602 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	23679..23903 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1748_RS25965 (18534)	18534..18761	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
F1748_RS25970 (18855)	18855..19541	-	687	WP_000332485.1	PAS domain-containing protein	-
F1748_RS25975 (19732)	19732..20115	-	384	WP_000124976.1	conjugal transfer relaxosome DNA-binding protein TraM	-
F1748_RS25980 (20392)	20392..21039	+	648	WP_000614935.1	transglycosylase SLT domain-containing protein	-
F1748_RS25985 (21336)	21336..22157	-	822	WP_001234469.1	DUF932 domain-containing protein	-
F1748_RS25990 (22278)	22278..22565	-	288	WP_000107535.1	hypothetical protein	-
F1748_RS25995 (22590)	22590..22796	-	207	WP_000547971.1	hypothetical protein	-
F1748_RS26000 (22866)	22866..23039	+	174	Protein_32	hypothetical protein	-
F1748_RS26005 (23477)	23477..23602	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
F1748_RS26010 (23544)	23544..23693	-	150	Protein_34	plasmid maintenance protein Mok	-
- (23681)	23681..23746	-	66	NuclAT_1	-	-
- (23679)	23679..23903	+	225	NuclAT_0	-	-
- (23679)	23679..23903	-	225	NuclAT_0	-	Antitoxin
- (23679)	23679..23903	-	225	NuclAT_0	-	Antitoxin
- (23679)	23679..23903	-	225	NuclAT_0	-	Antitoxin
- (23679)	23679..23903	-	225	NuclAT_0	-	Antitoxin
F1748_RS26015 (23715)	23715..23903	+	189	WP_001299721.1	hypothetical protein	-
F1748_RS26020 (23872)	23872..24634	-	763	Protein_36	plasmid SOS inhibition protein A	-
F1748_RS26025 (24631)	24631..25065	-	435	WP_089516080.1	conjugation system SOS inhibitor PsiB	-
F1748_RS26030 (25120)	25120..27078	-	1959	WP_089516078.1	ParB/RepB/Spo0J family partition protein	-
F1748_RS26035 (27140)	27140..27373	-	234	WP_000005974.1	DUF905 domain-containing protein	-
F1748_RS26040 (27430)	27430..27951	-	522	WP_122986597.1	single-stranded DNA-binding protein	-
F1748_RS26045 (28008)	28008..28398	+	391	Protein_41	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul3 / tet(A)	paa / hlyD / hlyB / hlyA / hlyC	1..114358	114358

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T139293 WP_001372321.1 NZ_CP043824:c23602-23477 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T139293 NZ_CP058618:3306287-3306814 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT139293 NZ_CP043824:c23903-23679 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin