Detailed information of TA system TA139282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4569047..4569649
• Replicon: chromosome
• Accession: NZ_CP043821
• Organism: Escherichia coli strain RM10740

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: F1748_RS22565
• Protein ID: WP_000897305.1
• Coordinates: 4569047..4569358 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F1748_RS22570
• Protein ID: WP_000356397.1
• Coordinates: 4569359..4569649 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1748_RS22535 (4564077)	4564077..4564862	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
F1748_RS22540 (4564961)	4564961..4565560	+	600	WP_001315111.1	glucose-1-phosphatase	-
F1748_RS22545 (4565554)	4565554..4566426	+	873	WP_000920762.1	virulence factor BrkB family protein	-
F1748_RS22550 (4566423)	4566423..4566860	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
F1748_RS22555 (4566905)	4566905..4567846	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
F1748_RS22560 (4567910)	4567910..4568818	-	909	WP_001162704.1	alpha/beta hydrolase	-
F1748_RS22565 (4569047)	4569047..4569358	+	312	WP_000897305.1	hypothetical protein	Toxin
F1748_RS22570 (4569359)	4569359..4569649	+	291	WP_000356397.1	NadS family protein	Antitoxin
F1748_RS22575 (4570254)	4570254..4570472	+	219	WP_001295676.1	CopG family transcriptional regulator	-
F1748_RS22580 (4570691)	4570691..4570933	+	243	WP_001086388.1	protein YiiF	-
F1748_RS22585 (4571263)	4571263..4572192	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
F1748_RS22590 (4572189)	4572189..4572824	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
F1748_RS22595 (4572821)	4572821..4573723	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T139282 WP_000897305.1 NZ_CP043821:4569047-4569358 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T139282 NZ_CP043821:4569047-4569358 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT139282 WP_000356397.1 NZ_CP043821:4569359-4569649 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT139282 NZ_CP058615:862841-863050 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTATCATGAATAATTTTGAAAGTTA
TGAACACACGGAATATGTTGATGGTATTGAAGTGGTTTCAGAGATCAGTCCTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAAAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7