Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3738794..3739593 Replicon chromosome
Accession NZ_CP043821
Organism Escherichia coli strain RM10740

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag F1748_RS18535 Protein ID WP_000347267.1
Coordinates 3739129..3739593 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag F1748_RS18530 Protein ID WP_001307405.1
Coordinates 3738794..3739129 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1748_RS18515 (3734579) 3734579..3735349 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
F1748_RS18520 (3735365) 3735365..3736699 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
F1748_RS18525 (3737074) 3737074..3738645 + 1572 WP_001273741.1 galactarate dehydratase -
F1748_RS18530 (3738794) 3738794..3739129 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
F1748_RS18535 (3739129) 3739129..3739593 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
F1748_RS18540 (3739648) 3739648..3740457 - 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
F1748_RS18545 (3740706) 3740706..3741986 + 1281 WP_000681947.1 tagatose-bisphosphate aldolase subunit KbaZ -
F1748_RS18550 (3742009) 3742009..3742482 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
F1748_RS18555 (3742493) 3742493..3743272 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
F1748_RS18560 (3743262) 3743262..3744140 + 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
F1748_RS18565 (3744158) 3744158..3744592 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3728254..3739593 11339


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T139279 WP_000347267.1 NZ_CP043821:3739129-3739593 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T139279 NZ_CP058615:731104-731208 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTTATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT139279 WP_001307405.1 NZ_CP043821:3738794-3739129 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT139279 NZ_CP058615:c731367-731203 [Staphylococcus aureus]
GTAGCAAGTAGAAGCAAAAGATGAAAATCATTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAAATGCCGGTCAAAGCGAATAGAAGGT
TATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References