Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 280151..280692 Replicon chromosome
Accession NZ_CP043821
Organism Escherichia coli strain RM10740

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag F1748_RS01250 Protein ID WP_000615976.1
Coordinates 280151..280429 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag F1748_RS01255 Protein ID WP_000729704.1
Coordinates 280432..280692 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1748_RS01235 (277733) 277733..278311 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
F1748_RS01240 (278517) 278517..279284 + 768 WP_000333380.1 class II glutamine amidotransferase -
F1748_RS01245 (279255) 279255..279995 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
F1748_RS01250 (280151) 280151..280429 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
F1748_RS01255 (280432) 280432..280692 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
F1748_RS01260 (280878) 280878..281651 + 774 WP_000543895.1 C40 family peptidase -
F1748_RS01265 (281708) 281708..282064 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
F1748_RS01270 (282057) 282057..282335 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
F1748_RS01275 (282440) 282440..284533 - 2094 WP_089515925.1 flagellar biosynthesis protein FlhA -
F1748_RS01280 (284517) 284517..285656 - 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 271747..282335 10588


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T139257 WP_000615976.1 NZ_CP043821:c280429-280151 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T139257 NZ_CP058596:4097180-4097557 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT139257 WP_000729704.1 NZ_CP043821:c280692-280432 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT139257 NZ_CP058596:4096759-4097133 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References