Detailed information of TA system TA139226

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 625996..626639
• Replicon: chromosome
• Accession: NZ_CP043772
• Organism: Haemophilus influenzae biotype aegyptius strain HE24/F3037

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5C2R1D6
• Locus tag: F1539_RS03195
• Protein ID: WP_041174909.1
• Coordinates: 625996..626334 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F1539_RS03200
• Protein ID: WP_006995911.1
• Coordinates: 626331..626639 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1539_RS03170	621564..621935	+	372	WP_013525751.1	hypothetical protein	-
F1539_RS03175	621925..622128	+	204	WP_006995916.1	hypothetical protein	-
F1539_RS03180	622115..622798	+	684	WP_013525750.1	hypothetical protein	-
F1539_RS03185	622791..623183	+	393	WP_006995914.1	hypothetical protein	-
F1539_RS03190	623171..625363	+	2193	WP_013525749.1	DUF927 domain-containing protein	-
F1539_RS03195	625996..626334	+	339	WP_041174909.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F1539_RS03200	626331..626639	+	309	WP_006995911.1	helix-turn-helix domain-containing protein	Antitoxin
F1539_RS03205	627052..627627	-	576	Protein_620	DDE-type integrase/transposase/recombinase	-
F1539_RS03210	627707..628381	-	675	WP_013525748.1	N-acylneuraminate cytidylyltransferase	-
F1539_RS03215	628495..629157	+	663	WP_013525747.1	NAD(P)H-dependent oxidoreductase	-
F1539_RS03220	629169..629342	+	174	Protein_623	antitoxin	-
F1539_RS03230	629455..629808	-	354	WP_006995906.1	type II toxin-antitoxin system PemK/MazF family toxin	-
F1539_RS03235	629808..630071	-	264	WP_171034549.1	antitoxin MazE	-
F1539_RS03240	630302..631414	-	1113	WP_013525746.1	iron-sulfur cluster carrier protein ApbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (11-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-80)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13150.10 Da
• Isoelectric Point: 9.2488

>T139226 WP_041174909.1 NZ_CP043772:625996-626334 [Haemophilus influenzae biotype aegyptius]
VKLSFIELPPFERYRKAHLSDDEYRAFQNELLENLEKGDVIQNAGGLRKIRIADSERNKGKRGGARVIYYYLIRKSQILL
VTAYSKNRCEDLTTEQYKILAKLVKEIEELEQ

Nucleotide

• Length: 339 bp

>T139226 NZ_CP058574:2550149-2550427 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCATCGACTTGCTGTGATTGATGCGGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11550.34 Da
• Isoelectric Point: 9.9619

>AT139226 WP_006995911.1 NZ_CP043772:626331-626639 [Haemophilus influenzae biotype aegyptius]
MSKLFDELIEGLNAYKDFTQGKITLRVETRERIEPASITAEEVKAIREKLNLSQAVFAQKLRTSVRTYQGWEQGKTKPSA
HASLLLRMVDKAPQTFELIAGI

Nucleotide

• Length: 309 bp

>AT139226 NZ_CP058574:2550427-2550711 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: A0A5C2R1D6