Detailed information of TA system TA139214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 489868..490511
• Replicon: chromosome
• Accession: NZ_CP043770
• Organism: Haemophilus influenzae biotype aegyptius strain HE7/F1946

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5C2R1D6
• Locus tag: F1537_RS02575
• Protein ID: WP_041174909.1
• Coordinates: 489868..490206 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F1537_RS02580
• Protein ID: WP_006995911.1
• Coordinates: 490203..490511 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1537_RS02550	485436..485807	+	372	WP_013525751.1	hypothetical protein	-
F1537_RS02555	485797..486000	+	204	WP_006995916.1	hypothetical protein	-
F1537_RS02560	485987..486670	+	684	WP_013525750.1	hypothetical protein	-
F1537_RS02565	486663..487055	+	393	WP_006995914.1	hypothetical protein	-
F1537_RS02570	487043..489235	+	2193	WP_013525749.1	DUF927 domain-containing protein	-
F1537_RS02575	489868..490206	+	339	WP_041174909.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F1537_RS02580	490203..490511	+	309	WP_006995911.1	helix-turn-helix domain-containing protein	Antitoxin
F1537_RS02585	490924..491499	-	576	Protein_498	DDE-type integrase/transposase/recombinase	-
F1537_RS02590	491579..492253	-	675	WP_013525748.1	N-acylneuraminate cytidylyltransferase	-
F1537_RS02595	492367..493029	+	663	WP_013525747.1	NAD(P)H-dependent oxidoreductase	-
F1537_RS02600	493041..493214	+	174	Protein_501	antitoxin	-
F1537_RS02610	493327..493680	-	354	WP_006995906.1	type II toxin-antitoxin system PemK/MazF family toxin	-
F1537_RS02615	493680..493943	-	264	WP_171034549.1	antitoxin MazE	-
F1537_RS02620	494174..495286	-	1113	WP_013525746.1	iron-sulfur cluster carrier protein ApbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (11-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-80)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13150.10 Da
• Isoelectric Point: 9.2488

>T139214 WP_041174909.1 NZ_CP043770:489868-490206 [Haemophilus influenzae biotype aegyptius]
VKLSFIELPPFERYRKAHLSDDEYRAFQNELLENLEKGDVIQNAGGLRKIRIADSERNKGKRGGARVIYYYLIRKSQILL
VTAYSKNRCEDLTTEQYKILAKLVKEIEELEQ

Nucleotide

• Length: 339 bp

>T139214 NZ_CP058571:c4685370-4685197 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCCTCTTTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11550.34 Da
• Isoelectric Point: 9.9619

>AT139214 WP_006995911.1 NZ_CP043770:490203-490511 [Haemophilus influenzae biotype aegyptius]
MSKLFDELIEGLNAYKDFTQGKITLRVETRERIEPASITAEEVKAIREKLNLSQAVFAQKLRTSVRTYQGWEQGKTKPSA
HASLLLRMVDKAPQTFELIAGI

Nucleotide

• Length: 309 bp

>AT139214 NZ_CP058571:c4685694-4685398 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A5C2R1D6