Detailed information of TA system TA139173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 336849..337440
• Replicon: chromosome
• Accession: NZ_CP043641
• Organism: Leifsonia shinshuensis strain INR9

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: F1C12_RS01665
• Protein ID: WP_185277148.1
• Coordinates: 336849..337130 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F1C12_RS01670
• Protein ID: WP_185277149.1
• Coordinates: 337156..337440 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1C12_RS01655	332379..333716	+	1338	WP_185277146.1	type I glutamate--ammonia ligase	-
F1C12_RS01660	333724..336759	+	3036	WP_185277147.1	bifunctional [glutamine synthetase] adenylyltransferase/[glutamine synthetase]-adenylyl-L-tyrosine phosphorylase	-
F1C12_RS01665	336849..337130	+	282	WP_185277148.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F1C12_RS01670	337156..337440	+	285	WP_185277149.1	HigA family addiction module antidote protein	Antitoxin
F1C12_RS01675	337500..337811	-	312	WP_185277150.1	hypothetical protein	-
F1C12_RS01680	338116..339447	+	1332	WP_185277151.1	LCP family protein	-
F1C12_RS01685	339464..339973	-	510	WP_185277152.1	hypothetical protein	-
F1C12_RS01690	340071..341168	-	1098	WP_185277153.1	acyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (20-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (2-91)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10741.34 Da
• Isoelectric Point: 8.5393

>T139173 WP_185277148.1 NZ_CP043641:336849-337130 [Leifsonia shinshuensis]
MLQSFVDRDAEAVWLGLPSRRIHPDIHRIARRKLAMLNAAAVLEDCRVPPGNRLEKLRGDRVGRYSIRINDQFRICFTWT
EGGPADVEIVDYH

Nucleotide

• Length: 282 bp

>T139173 NZ_CP058548:c102903-102801 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10227.70 Da
• Isoelectric Point: 4.7541

>AT139173 WP_185277149.1 NZ_CP043641:337156-337440 [Leifsonia shinshuensis]
MTDPITPGEILSEEFMAPLGLTKYALAKAVHVPAQRIGDIVNGKRAITPDTALRFARAFGTTAEFWLNLQAHHDIELALA
MHSDEFDEIAPLAG

Nucleotide

• Length: 285 bp

>AT139173 NZ_CP058548:102794-102939 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT