Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1326020..1326617 Replicon chromosome
Accession NZ_CP043597
Organism Klebsiella pneumoniae strain WCHKP085031

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag B7D33_RS06415 Protein ID WP_004142563.1
Coordinates 1326020..1326337 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag B7D33_RS06420 Protein ID WP_004142561.1
Coordinates 1326330..1326617 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D33_RS06400 (1321723) 1321723..1323150 - 1428 WP_004176980.1 MFS transporter -
B7D33_RS06405 (1323259) 1323259..1324125 + 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
B7D33_RS06410 (1324792) 1324792..1325835 + 1044 WP_004178895.1 DUF2157 domain-containing protein -
B7D33_RS06415 (1326020) 1326020..1326337 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B7D33_RS06420 (1326330) 1326330..1326617 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
B7D33_RS06425 (1326882) 1326882..1327535 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
B7D33_RS06430 (1327655) 1327655..1328023 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
B7D33_RS06435 (1328023) 1328023..1328604 - 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
B7D33_RS06440 (1328784) 1328784..1329899 + 1116 WP_020316605.1 MBL fold metallo-hydrolase -
B7D33_RS06445 (1329930) 1329930..1330271 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
B7D33_RS06450 (1330289) 1330289..1330537 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T139135 WP_004142563.1 NZ_CP043597:1326020-1326337 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T139135 NZ_CP058362:54140-54622 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT139135 WP_004142561.1 NZ_CP043597:1326330-1326617 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT139135 NZ_CP058362:53886-54152 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References