Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 2398549..2399238 Replicon chromosome
Accession NZ_CP043590
Organism Klebsiella pneumoniae strain WCHKP040009

Toxin (Protein)


Gene name higB Uniprot ID A0A331C6E2
Locus tag C6M58_RS11730 Protein ID WP_021469727.1
Coordinates 2398549..2398866 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6B0N7G3
Locus tag C6M58_RS11735 Protein ID WP_020804705.1
Coordinates 2398942..2399238 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M58_RS11700 (2394251) 2394251..2394760 + 510 WP_020802144.1 GNAT family N-acetyltransferase -
C6M58_RS11705 (2394770) 2394770..2395696 + 927 WP_032435210.1 amino acid ABC transporter permease -
C6M58_RS11710 (2395680) 2395680..2396459 + 780 WP_002906697.1 amino acid ABC transporter ATP-binding protein -
C6M58_RS11715 (2396498) 2396498..2397349 + 852 WP_072198177.1 transporter substrate-binding domain-containing protein -
C6M58_RS11720 (2397427) 2397427..2398044 + 618 WP_032425015.1 glutathione S-transferase family protein -
C6M58_RS11725 (2398115) 2398115..2398342 + 228 WP_002906690.1 tautomerase PptA -
C6M58_RS11730 (2398549) 2398549..2398866 + 318 WP_021469727.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6M58_RS11735 (2398942) 2398942..2399238 + 297 WP_020804705.1 NadS family protein Antitoxin
C6M58_RS11740 (2399317) 2399317..2399763 + 447 WP_032435212.1 hypothetical protein -
C6M58_RS11745 (2399804) 2399804..2401420 - 1617 WP_004175961.1 carbohydrate porin -
C6M58_RS11750 (2401464) 2401464..2402846 - 1383 WP_004151222.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12221.10 Da        Isoelectric Point: 10.2217

>T139122 WP_021469727.1 NZ_CP043590:2398549-2398866 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Download         Length: 318 bp

>T139122 NZ_CP058358:c5374494-5374111 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11057.93 Da        Isoelectric Point: 6.7277

>AT139122 WP_020804705.1 NZ_CP043590:2398942-2399238 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMLMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRHPTGAARK
LLLLLQGNPLIINLLKVV

Download         Length: 297 bp

>AT139122 NZ_CP058358:c5374736-5374494 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331C6E2

Antitoxin

Source ID Structure
AlphaFold DB A0A6B0N7G3

References