Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 331725..332311 Replicon chromosome
Accession NZ_CP043585
Organism Klebsiella pneumoniae subsp. ozaenae strain SCKP020151

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag CLQ49_RS01545 Protein ID WP_002920800.1
Coordinates 331943..332311 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag CLQ49_RS01540 Protein ID WP_004174006.1
Coordinates 331725..331946 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ49_RS01520 (CLQ49_01530) 327882..328808 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
CLQ49_RS01525 (CLQ49_01535) 328805..330082 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
CLQ49_RS01530 (CLQ49_01540) 330079..330846 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
CLQ49_RS01535 (CLQ49_01545) 330848..331561 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
CLQ49_RS01540 (CLQ49_01550) 331725..331946 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CLQ49_RS01545 (CLQ49_01555) 331943..332311 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CLQ49_RS01550 (CLQ49_01560) 332584..333900 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
CLQ49_RS01555 (CLQ49_01565) 334007..334894 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
CLQ49_RS01560 (CLQ49_01570) 334891..335736 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
CLQ49_RS01565 (CLQ49_01575) 335738..336808 + 1071 WP_017880090.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 326711..337545 10834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T139074 WP_002920800.1 NZ_CP043585:331943-332311 [Klebsiella pneumoniae subsp. ozaenae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T139074 NZ_CP058350:c1997857-1997609 [Rhizobium sp. ADMK78]
ATGATCATCGTTTGGGATGAACCGAAGCGGCTGCAGAATTTGGCAAAGCACGGATTGGATTTTGCCGAGCTCGATGAGAG
CTTTTTCGTCGATGCCAGCATCCGTGCGGCGAAATCGGGGCGGACGATCGCGATCGGCGAATTGAACGGTCGGATCGTCG
TTGCCGTAGTCTATACGCCACTGGGTCGCGAAGCATTGTCGATCATATCCATGCGGCCAGCCAGCCGGCGGGAAAGGAGC
CTGATATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT139074 WP_004174006.1 NZ_CP043585:331725-331946 [Klebsiella pneumoniae subsp. ozaenae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT139074 NZ_CP058350:c1997612-1997301 [Rhizobium sp. ADMK78]
ATGAGCGGAAAATTTTCGTCCAAGAGGCCGCTGACCGACGAAGAAGAGGCCGAGATCCAGGCGATGATCGCCGCCGACCC
CGACAATCCCGAAGTGACCGACGAGGAGTTGAAGGAGTTCCGACCATTCCGTGAGGTCTTTCCTGATCTCGCCGAGGCGA
TTGACCGCAGGCTCGGCCGACCGAAGGCGGAGAGCCCGAAGAAGGCGATCAGCATCCGGCTTGATCAGGAGGTGATCGAT
CGGTTCAAGGCAACGGGCGATGGCTGGCAGTCGCGGATGAACGAGGCGCTGCGCAAGGCGGTGGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References