Detailed information of TA system TA139069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 57413..58140
• Replicon: plasmid pVir_020018
• Accession: NZ_CP043581
• Organism: Klebsiella pneumoniae strain SCKP020018

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: C6M74_RS26550
• Protein ID: WP_011251285.1
• Coordinates: 57829..58140 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6M74_RS26545
• Protein ID: WP_011251286.1
• Coordinates: 57413..57832 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6M74_RS26515 (C6M74_26800)	52581..53051	-	471	WP_048333570.1	hypothetical protein	-
C6M74_RS26520	53364..53999	-	636	WP_223171879.1	hypothetical protein	-
C6M74_RS26525 (C6M74_26810)	54418..55038	+	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
C6M74_RS26530 (C6M74_26815)	55059..55847	+	789	WP_040217257.1	hypothetical protein	-
C6M74_RS26535 (C6M74_26820)	55861..56226	+	366	WP_048333448.1	hypothetical protein	-
C6M74_RS26540 (C6M74_26825)	56298..57266	-	969	WP_086642568.1	IS5 family transposase	-
C6M74_RS26545 (C6M74_26835)	57413..57832	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
C6M74_RS26550 (C6M74_26840)	57829..58140	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
C6M74_RS26555 (C6M74_26845)	58345..58782	-	438	Protein_58	DDE-type integrase/transposase/recombinase	-
C6M74_RS26560 (C6M74_26850)	58917..59614	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
C6M74_RS26565 (C6M74_26855)	61000..61650	-	651	WP_068893702.1	DUF1173 family protein	-
C6M74_RS26570	61683..61949	-	267	WP_223175074.1	DUF1173 family protein	-
C6M74_RS26575 (C6M74_26860)	62127..62621	+	495	WP_004212794.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA	1..229186	229186
	-	inside	IScluster/Tn	-	-	47292..59614	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T139069 WP_011251285.1 NZ_CP043581:c58140-57829 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T139069 NZ_CP058350:360365-360658 [Rhizobium sp. ADMK78]
GTGCCACGAGTAGTCGTGACTGAGGGAGCGGCGATTGGCTTGGAGCGATGCCGTCGTTTCCTTCAGGCTAAGTCACTGGA
TGCCGCAGAGAAGGCGGCCAAGGAAATAAAGAGACAGTTCACGCTGCTTGCAAGCAATCCGAAACGGGGGCGGCCCCTGC
CGCGCTTCCCGGACCTGCGGGAACTGACGATCGGGTTCGGCGCGGCCGGCTATGTCGCTCTTTATCGCTACGATGAACCG
GAAGATGTTGTCCACATCCTGGCATTCAAACATCAGAAAGAGGCCGGCTACTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT139069 WP_011251286.1 NZ_CP043581:c57832-57413 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT139069 NZ_CP058350:360105-360377 [Rhizobium sp. ADMK78]
ATGGCGACCTCGCTGAAAATCGATGACGCATTGAAAGAGCGGGTGCAGCAACTTGCCAACGAGCGCAAACGCTCGGCGCA
TGGACTGATGCTGGAAGCGATTGAGCAATATGTGGAGCGTGAAGAGGCGCGTGAAAGCTTCACCCGTGAAGCGCTGGCAT
CGTGGACTGCTTACAAGGAAACGGGTCGCCATCTGACACTGGACGAGGTGAACGACTGGCTCGAAACCTGGGGCTCAGCC
GACGAAAAACCGGTGCCCGAGTGCCACGAGTAG

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1