Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 3556163..3556863 Replicon chromosome
Accession NZ_CP043576
Organism Pseudomonas putida strain T25-27

Toxin (Protein)


Gene name mqsR Uniprot ID A0A2I0CDW7
Locus tag F0Q09_RS16225 Protein ID WP_003254238.1
Coordinates 3556163..3556459 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID I7AUJ7
Locus tag F0Q09_RS16230 Protein ID WP_003254235.1
Coordinates 3556462..3556863 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F0Q09_RS16205 3551799..3553424 + 1626 WP_003254243.1 twin-arginine translocation signal domain-containing protein -
F0Q09_RS16210 3553546..3554022 + 477 WP_064614924.1 sigma-70 family RNA polymerase sigma factor -
F0Q09_RS16215 3554191..3554670 + 480 WP_003254240.1 hypothetical protein -
F0Q09_RS16220 3554743..3556068 + 1326 WP_182374793.1 MFS transporter -
F0Q09_RS16225 3556163..3556459 + 297 WP_003254238.1 type II toxin-antitoxin system MqsR family toxin Toxin
F0Q09_RS16230 3556462..3556863 + 402 WP_003254235.1 type II toxin-antitoxin system MqsA family antitoxin Antitoxin
F0Q09_RS16235 3556935..3558617 - 1683 WP_023389616.1 electron transfer flavoprotein-ubiquinone oxidoreductase -
F0Q09_RS16240 3559153..3559902 + 750 WP_003254232.1 electron transfer flavoprotein subunit beta/FixA family protein -
F0Q09_RS16245 3559904..3560845 + 942 WP_004573654.1 FAD-binding protein -
F0Q09_RS16250 3560921..3561742 + 822 WP_003254229.1 transporter substrate-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(6-97)

Antitoxin

Cro/C1-type helix-turn-helix domain

(74-109)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11110.18 Da        Isoelectric Point: 10.2270

>T139046 WP_003254238.1 NZ_CP043576:3556163-3556459 [Pseudomonas putida]
MEKRMPNCPLERVKALAAARRIRPTGAALKGAKALGMDFPGMLEVITSLKRTDFYKSMTSHIDHRVWQDVYRPLTAIGYV
YLKLSVVDDVLIVSFKEL

Download         Length: 297 bp

>T139046 NZ_CP058342:3535564-3535782 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14684.76 Da        Isoelectric Point: 4.9531

>AT139046 WP_003254235.1 NZ_CP043576:3556462-3556863 [Pseudomonas putida]
MRCPICGGSELAPDIQGMPYSYKGEMTVIPEVSGDYCSACGECVLSHDEAMRVSHLMTAFERQVNANVVDPSFIASIRRK
FDLDQREAGEIFGGGVNAFSRYENGKTTPPVALVKLLKLLDRHPELFEEVRTA

Download         Length: 402 bp

>AT139046 NZ_CP058342:3535164-3535538 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I0CDW7

Antitoxin

Source ID Structure
AlphaFold DB I7AUJ7

References