Detailed information of TA system TA139024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3616736..3617333
• Replicon: chromosome
• Accession: NZ_CP043562
• Organism: Klebsiella pneumoniae strain P094-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: F0H50_RS17965
• Protein ID: WP_004893639.1
• Coordinates: 3617016..3617333 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: F0H50_RS17960
• Protein ID: WP_004142561.1
• Coordinates: 3616736..3617023 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0H50_RS17930	3612816..3613064	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
F0H50_RS17935	3613082..3613423	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
F0H50_RS17940	3613454..3614569	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
F0H50_RS17945	3614749..3615333	+	585	WP_002893026.1	TetR/AcrR family transcriptional regulator	-
F0H50_RS17950	3615330..3615698	+	369	WP_002893024.1	MmcQ/YjbR family DNA-binding protein	-
F0H50_RS17955	3615818..3616471	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
F0H50_RS17960	3616736..3617023	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
F0H50_RS17965	3617016..3617333	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F0H50_RS17970	3617519..3618535	-	1017	WP_149362513.1	DUF2157 domain-containing protein	-
F0H50_RS17975	3619226..3620087	-	862	Protein_3491	helix-turn-helix domain-containing protein	-
F0H50_RS17980	3620187..3620921	-	735	Protein_3492	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T139024 WP_004893639.1 NZ_CP043562:c3617333-3617016 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T139024 NZ_CP058333:459637-459819 [Pseudomonas aeruginosa]
ATGCGGAGTAGGGAAGTGATTGATTTGCTGTTGGAGGATGGCTGGTATGAAGTGGCGGTGAAAGGTAGCCACCACCAGTT
CAAACATCCTTCAAAGCCTGGAAAGGTGACGGTTCAACATCCGTCCTCGACGATACCGAAAGGAACCCTGAACAACATAC
TGAAACAAGCCGGCCTCAAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT139024 WP_004142561.1 NZ_CP043562:c3617023-3616736 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT139024 NZ_CP043562:c3617023-3616736 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACGTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1