Detailed information of TA system TA139004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1028715..1029330
• Replicon: chromosome
• Accession: NZ_CP043556
• Organism: Vibrio cholerae strain RFB16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: F0316_RS19090
• Protein ID: WP_001162670.1
• Coordinates: 1028715..1029002 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F0316_RS19095
• Protein ID: WP_001232701.1
• Coordinates: 1029013..1029330 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0316_RS19050	1023808..1024233	+	426	WP_000620001.1	N-acetyltransferase	-
F0316_RS19055	1025188..1025535	+	348	WP_000933409.1	hypothetical protein	-
F0316_RS19060	1025831..1026010	+	180	WP_149560837.1	DUF645 family protein	-
F0316_RS19070	1026268..1026678	+	411	WP_149560879.1	dual specificity protein phosphatase family protein	-
F0316_RS19075	1026915..1027832	+	918	WP_172626104.1	DUF2971 domain-containing protein	-
F0316_RS19080	1028089..1028487	+	399	WP_021706447.1	VOC family protein	-
F0316_RS19090	1028715..1029002	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F0316_RS19095	1029013..1029330	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
F0316_RS19105	1029623..1029804	+	182	Protein_933	DUF645 family protein	-
F0316_RS19115	1030002..1030427	+	426	WP_149560839.1	hypothetical protein	-
F0316_RS19120	1030527..1030775	+	249	Protein_935	DUF3709 domain-containing protein	-
F0316_RS19125	1031045..1031521	+	477	WP_001047183.1	GNAT family N-acetyltransferase	-
F0316_RS19135	1031640..1032155	+	516	WP_000090010.1	hypothetical protein	-
F0316_RS19140	1032293..1032637	+	345	Protein_938	hypothetical protein	-
F0316_RS19145	1032697..1033737	-	1041	WP_057569038.1	IS481 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1032697..1033737	1040
	-	inside	Integron	-	-	981561..1071623	90062

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T139004 WP_001162670.1 NZ_CP043556:1028715-1029002 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T139004 NZ_CP058325:c4938345-4937998 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT139004 WP_001232701.1 NZ_CP043556:1029013-1029330 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT139004 NZ_CP058325:c4937998-4937699 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36