Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3982288..3982829 Replicon chromosome
Accession NZ_CP043542
Organism Escherichia coli strain F2_81

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag CPH39_RS19310 Protein ID WP_096220693.1
Coordinates 3982551..3982829 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CPH39_RS19305 Protein ID WP_000729704.1
Coordinates 3982288..3982548 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPH39_RS19280 (3978071) 3978071..3978856 - 786 WP_000207551.1 putative lateral flagellar export/assembly protein LafU -
CPH39_RS19285 (3978828) 3978828..3980540 + 1713 Protein_3800 flagellar biosynthesis protein FlhA -
CPH39_RS19290 (3980645) 3980645..3980923 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
CPH39_RS19295 (3980916) 3980916..3981272 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
CPH39_RS19300 (3981329) 3981329..3982078 - 750 WP_053884036.1 C40 family peptidase -
CPH39_RS19305 (3982288) 3982288..3982548 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CPH39_RS19310 (3982551) 3982551..3982829 + 279 WP_096220693.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CPH39_RS19315 (3982985) 3982985..3983725 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
CPH39_RS19320 (3983696) 3983696..3984463 - 768 WP_000333380.1 class II glutamine amidotransferase -
CPH39_RS19325 (3984669) 3984669..3985247 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3912242..3983725 71483


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10844.60 Da        Isoelectric Point: 9.9107

>T138991 WP_096220693.1 NZ_CP043542:3982551-3982829 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHNDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAVLFG

Download         Length: 279 bp

>T138991 NZ_CP058321:c2136936-2136658 [Halomonas sp. SH5A2]
ATGATTAAATCATTCCGTCACAAAGGACTGAAACGGTTCTACTCGACCGGCAACACGTCTGGCATCCAGCCGGATCACGC
CAAGAAACTGCGTATGCAGTTGGCCGCTCTGGATACCGCCACTGCGGTGGAGGACATGGAGATCCCTGGTTTTCGGCTTC
ATCCATTGAAGGGTAAAGACAAAGGGCGGTGGTCGATTCGGGTCAACGGAAACTGGCGCATAACGTTCGAATTTCAGGAC
GGCAACGCCTACATTCTTGATTATGAGGATTATCACTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT138991 WP_000729704.1 NZ_CP043542:3982288-3982548 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT138991 NZ_CP058321:c2136658-2136365 [Halomonas sp. SH5A2]
ATGACTATGCATAATCCGCCGCATCCCGGTGAGTTCATTCGGGAGGTTTACCTTGAGCCTTTCGGTGTCAGTTCTCGTCA
GCTCGCGTCCAGTCTGGGGGTCTCGCCTTCCACGTTGTCTCGGTTGCTCAAAGGGGATAGTGGTATTAGCCCGGAAATGT
CGCTTCGGCTATCCAAGGTTCTCGGGCGTACTCCTGAGAGCTGGTTGGCGATGCAGGACATGTACGATCTTTGGGTGGCC
CGCAACACGGTCAACCTGGATGGAATCCATCCCCTGGACTTCGAGGCAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References