Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2475855..2476417 Replicon chromosome
Accession NZ_CP043542
Organism Escherichia coli strain F2_81

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag CPH39_RS12045 Protein ID WP_000605676.1
Coordinates 2475855..2476133 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag CPH39_RS12050 Protein ID WP_000781370.1
Coordinates 2476133..2476417 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPH39_RS12020 (2471441) 2471441..2471872 - 432 WP_000152305.1 peroxiredoxin OsmC -
CPH39_RS12025 (2472217) 2472217..2472432 + 216 WP_096220623.1 biofilm-dependent modulation protein -
CPH39_RS12030 (2472534) 2472534..2472671 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
CPH39_RS12035 (2472828) 2472828..2474525 + 1698 WP_000433464.1 malate dehydrogenase -
CPH39_RS12040 (2474659) 2474659..2475669 + 1011 WP_000642406.1 alcohol dehydrogenase AdhP -
CPH39_RS12045 (2475855) 2475855..2476133 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CPH39_RS12050 (2476133) 2476133..2476417 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
CPH39_RS12055 (2476468) 2476468..2477121 - 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
CPH39_RS12060 (2477114) 2477114..2477998 - 885 WP_001240582.1 formate dehydrogenase N subunit beta -
CPH39_RS12065 (2478011) 2478011..2481058 - 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T138986 WP_000605676.1 NZ_CP043542:2475855-2476133 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T138986 NZ_CP058315:2265107-2265382 [Treponema parvum]
ATGAAATATAAATTTGATTATACAACCCGATTTAAAAAAGACTATCGTAAGCTTATCGATCAAAGCGTTATTGATGAGGT
TGACACGGTTATCGAAAAACTTTTAAACGGCAAACTCCTTGAAAAAAAGTATAAGGATCATGCATTAAGCGGAAGCTATA
AGGGTTGCCGTGATTGTCATATACGCCCCGATTTAGTATTAATTTATAAAAAAAATAATGAAATTTTGGTTTTGACCGCT
TTAAGGGTAGGCACGCACAGCGAGTTATTCTATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT138986 WP_000781370.1 NZ_CP043542:2476133-2476417 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT138986 NZ_CP058315:2264827-2265117 [Treponema parvum]
ATGGCAAAGGCGGCAAGTTTCAGCATAAGAATGGATTCCGACATAAAAAAACAAAGCGAATCTTTGTATAATGCACTCGG
CTTGAATTTGACGACGGCAATCAATGTGTTCCTGAGGAAGTCTATACAAGCCGGCGGCTTTCCTTTTGATGTACGACTGG
ACAACTTTAACAACGAAACCGTACAGGCAATAAAAGAAGCTGATTTCATAGCTGAGGAAATTAAAAGCGGGCGCAGAAAG
CCTTTCACCTCTTGGGAAAAAGCAAAAAAAAGCTTGATCAATGAAATATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References