Detailed information of TA system TA138970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 607711..608510
• Replicon: chromosome
• Accession: NZ_CP043542
• Organism: Escherichia coli strain F2_81

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: CPH39_RS02910
• Protein ID: WP_048969505.1
• Coordinates: 607711..608175 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: CPH39_RS02915
• Protein ID: WP_001307405.1
• Coordinates: 608175..608510 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPH39_RS02880 (602712)	602712..603146	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
CPH39_RS02885 (603164)	603164..604042	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
CPH39_RS02890 (604032)	604032..604811	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
CPH39_RS02895 (604822)	604822..605295	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CPH39_RS02900 (605318)	605318..606598	-	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CPH39_RS02905 (606847)	606847..607656	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CPH39_RS02910 (607711)	607711..608175	-	465	WP_048969505.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CPH39_RS02915 (608175)	608175..608510	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CPH39_RS02920 (608659)	608659..610230	-	1572	WP_001273763.1	galactarate dehydratase	-
CPH39_RS02925 (610605)	610605..611939	+	1335	WP_000599633.1	galactarate/glucarate/glycerate transporter GarP	-
CPH39_RS02930 (611955)	611955..612725	+	771	WP_024250085.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17862.33 Da
• Isoelectric Point: 9.6924

>T138970 WP_048969505.1 NZ_CP043542:c608175-607711 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFIKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T138970 NZ_CP058310:c50638-50480 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT138970 WP_001307405.1 NZ_CP043542:c608510-608175 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT138970 NZ_CP058310:c50906-50682 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin

• AlphaFold DB: A0A0E0XVC7