Detailed information of TA system TA138949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2137325..2138019
• Replicon: chromosome
• Accession: NZ_CP043539
• Organism: Escherichia coli O157 strain Al Ain

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: F0L19_RS11120
• Protein ID: WP_001263495.1
• Coordinates: 2137325..2137723 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: F0L19_RS11125
• Protein ID: WP_000554757.1
• Coordinates: 2137726..2138019 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	2132912..2132992	-	81	NuclAT_11	-	-
-	2132912..2132992	-	81	NuclAT_11	-	-
-	2132912..2132992	-	81	NuclAT_11	-	-
-	2132912..2132992	-	81	NuclAT_11	-	-
F0L19_RS11095	2133588..2134046	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
F0L19_RS11100	2134307..2135764	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
F0L19_RS11105	2135821..2136342	-	522	Protein_2100	peptide chain release factor H	-
F0L19_RS11110	2136341..2136544	-	204	Protein_2101	RNA ligase RtcB family protein	-
F0L19_RS11115	2136863..2137315	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
F0L19_RS11120	2137325..2137723	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
F0L19_RS11125	2137726..2138019	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
F0L19_RS11130	2138071..2139126	-	1056	WP_001226186.1	DNA polymerase IV	-
F0L19_RS11135	2139197..2139982	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
F0L19_RS11140	2139927..2141666	+	1740	WP_001301901.1	flagellar biosynthesis protein FlhA	-
F0L19_RS11145	2141811..2142308	-	498	Protein_2108	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T138949 WP_001263495.1 NZ_CP043539:c2137723-2137325 [Escherichia coli O157]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T138949 NZ_CP058308:932676-933083 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT138949 WP_000554757.1 NZ_CP043539:c2138019-2137726 [Escherichia coli O157]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT138949 NZ_CP058308:932429-932695 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTAATCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: S1FJN6