Detailed information of TA system TA138927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 3307460..3308911
• Replicon: chromosome
• Accession: NZ_CP043538
• Organism: Methylobacterium mesophilicum SR1.6/6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MMSR116_RS15685
• Protein ID: WP_010682165.1
• Coordinates: 3308573..3308911 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MMSR116_RS15680
• Protein ID: WP_010682166.1
• Coordinates: 3307460..3308554 (-)
• Length: 365 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMSR116_RS15660	3303064..3303642	-	579	WP_010682170.1	hypothetical protein	-
MMSR116_RS15665	3303639..3305360	-	1722	WP_010682169.1	SIR2 family protein	-
MMSR116_RS15670	3305633..3306061	+	429	WP_010682168.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
MMSR116_RS15675	3306058..3307344	+	1287	WP_010682167.1	Y-family DNA polymerase	-
MMSR116_RS15680	3307460..3308554	-	1095	WP_010682166.1	HigA family addiction module antidote protein	Antitoxin
MMSR116_RS15685	3308573..3308911	-	339	WP_010682165.1	system killer suppression protein	Toxin
MMSR116_RS15690	3309077..3309280	+	204	WP_010682164.1	CsbD family protein	-
MMSR116_RS15700	3309871..3310731	+	861	WP_010682162.1	DNA starvation/stationary phase protection protein	-
MMSR116_RS15705	3311099..3311644	+	546	WP_010682161.1	cytochrome b	-
MMSR116_RS15710	3311650..3312345	+	696	WP_010682160.1	VIT family protein	-
MMSR116_RS15715	3312346..3312594	-	249	WP_010682159.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-68)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12672.51 Da
• Isoelectric Point: 10.0933

>T138927 WP_010682165.1 NZ_CP043538:c3308911-3308573 [Methylobacterium mesophilicum SR1.6/6]
MKIRFRSRRLEKDLSDAKPMAKAYGDRAKPIRRRLDVLRLAACLSDVPTGPPDHRHQLGNNRNGQLSVTIKDNWRIVFLP
DHDPVPRLPDGGLDTGQVTAVEIIEIVDYHGN

Nucleotide

• Length: 339 bp

>T138927 NZ_CP058302:4229618-4230001 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 365 a.a.
• Molecular weight: 40892.61 Da
• Isoelectric Point: 6.5480

>AT138927 WP_010682166.1 NZ_CP043538:c3308554-3307460 [Methylobacterium mesophilicum SR1.6/6]
MTTQENWAPQWATHPGEHLEEYIQERGWSQAEFARIADMTPKLVSTIINGSNPVTPETAIKLEHVLGLKAYIWTGLQANW
DLFQAREREREAAAREAEWLKGFPVRELKACGRIPQTTDPGGIMDGLLGLLGIGTPHAYEAKLGSLAVCHRRAKAGTTSP
HHMLCWLMLGEEQARRLSLPAYDPDRFVEGCHEIRALTVERPETFEPRMKQLCHAAGVALVFQKPLSKTCVFGSARWMND
ERPLIQMSLRMKSNDHFWWTFFHEAAHIVLHRGRNFADDQNGEGDHVEAEADAWAEEVLVGRERIGAFATTCPGTKQDIV
AFAGEVGLHPGIVVGMLQHRRIVPFSHLNDLKVRFAWADEVATA

Nucleotide

• Length: 1095 bp

>AT138927 NZ_CP058302:4229157-4229537 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG