Detailed information of TA system TA138923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4719179..4719909
• Replicon: chromosome
• Accession: NZ_CP043511
• Organism: Enterobacter kobei strain EB_P8_L5_01.19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2W0GI63
• Locus tag: FZO55_RS23125
• Protein ID: WP_023331686.1
• Coordinates: 4719595..4719909 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FZO55_RS23120
• Protein ID: WP_127341140.1
• Coordinates: 4719179..4719598 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZO55_RS23095	4714667..4716172	-	1506	WP_014881932.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
FZO55_RS23100	4716169..4716762	-	594	WP_014881931.1	transcriptional regulator UhpA	-
FZO55_RS23105	4716845..4717132	-	288	WP_010426528.1	acetolactate synthase small subunit	-
FZO55_RS23110	4717136..4718824	-	1689	WP_047028139.1	acetolactate synthase large subunit	-
FZO55_RS23115	4718931..4719029	-	99	WP_023331688.1	ilvB operon leader peptide IvbL	-
FZO55_RS23120	4719179..4719598	-	420	WP_127341140.1	helix-turn-helix domain-containing protein	Antitoxin
FZO55_RS23125	4719595..4719909	-	315	WP_023331686.1	type II toxin-antitoxin system HigB family toxin	Toxin
FZO55_RS23130	4720257..4721476	+	1220	Protein_4444	ISL3-like element ISKox3 family transposase	-
FZO55_RS23135	4721720..4721809	+	90	WP_023331685.1	type I toxin-antitoxin system toxin TisB	-
FZO55_RS23140	4721874..4722320	+	447	WP_014881927.1	GNAT family N-acetyltransferase	-
FZO55_RS23145	4722383..4723216	+	834	WP_045134488.1	DMT family transporter	-
FZO55_RS23150	4723392..4724576	+	1185	WP_045269345.1	multidrug efflux MFS transporter EmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4710789..4720769	9980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12441.59 Da
• Isoelectric Point: 10.2825

>T138923 WP_023331686.1 NZ_CP043511:c4719909-4719595 [Enterobacter kobei]
MHLISMKAILDAVIQFPQHREELLFLGRTIEKSHCTTPVALRKIFPTLDNLKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T138923 NZ_CP058302:c3312772-3312494 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15533.13 Da
• Isoelectric Point: 5.6761

>AT138923 WP_127341140.1 NZ_CP043511:c4719598-4719179 [Enterobacter kobei]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMASRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT138923 NZ_CP058302:c3313035-3312775 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A2W0GI63