Detailed information of TA system TA138919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3645289..3645938
• Replicon: chromosome
• Accession: NZ_CP043511
• Organism: Enterobacter kobei strain EB_P8_L5_01.19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FZO55_RS17860
• Protein ID: WP_023332294.1
• Coordinates: 3645289..3645651 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2W0GCS7
• Locus tag: FZO55_RS17865
• Protein ID: WP_014882863.1
• Coordinates: 3645639..3645938 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZO55_RS17840	3640574..3641596	-	1023	WP_058690610.1	LacI family transcriptional regulator	-
FZO55_RS17845	3641672..3642814	-	1143	WP_058690611.1	Gfo/Idh/MocA family oxidoreductase	-
FZO55_RS17850	3642807..3643832	-	1026	WP_032637265.1	sugar phosphate isomerase/epimerase	-
FZO55_RS17855	3643848..3645083	-	1236	WP_023332295.1	MFS transporter	-
FZO55_RS17860	3645289..3645651	+	363	WP_023332294.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FZO55_RS17865	3645639..3645938	+	300	WP_014882863.1	helix-turn-helix transcriptional regulator	Antitoxin
FZO55_RS17870	3646049..3647437	-	1389	WP_058690612.1	phenylalanine transporter	-
FZO55_RS17875	3647540..3649512	-	1973	Protein_3433	PhoX family phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-80)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13953.90 Da
• Isoelectric Point: 6.2260

>T138919 WP_023332294.1 NZ_CP043511:3645289-3645651 [Enterobacter kobei]
MWNVETTDRFDQWYFEQTTALKEDVLAMMHILAEFGPTLGRPYVDTVKASAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYNEMITLADVEYSRHLADKEAIWQR

Nucleotide

• Length: 363 bp

>T138919 NZ_CP058302:c2062461-2062354 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10860.61 Da
• Isoelectric Point: 9.8627

>AT138919 WP_014882863.1 NZ_CP043511:3645639-3645938 [Enterobacter kobei]
MATLKELMAQQSAESQERIAAKVDVIRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT138919 NZ_CP043511:3645639-3645938 [Enterobacter kobei]
ATGGCAACGCTAAAAGAACTCATGGCACAACAGAGTGCGGAAAGTCAGGAGCGTATTGCGGCTAAAGTTGATGTAATACG
CAGAATTGTTGCCCTGAACCAACTGCGGGAAGAGCTCAATATTTCGCAGACAGAACTGGCCGCGGCAATGGGGGTAAAAC
AACCAACTGTGGCAAAAATCGAACAGCCGGGAAACGATCCCCGGCTTTCAACACTCAAACGCTATGTCAGTGCGCTCGGC
GGTGAAATGAGCATTGATGTGACGTTACCGAATGGGAAACGAATAGCGTTTCAGATTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2W0GCS7