Detailed information of TA system TA138884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4192167..4192769
• Replicon: chromosome
• Accession: NZ_CP043487
• Organism: Escherichia coli strain AQ15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FQT78_RS20220
• Protein ID: WP_000897305.1
• Coordinates: 4192167..4192478 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FQT78_RS20225
• Protein ID: WP_000356397.1
• Coordinates: 4192479..4192769 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQT78_RS20190	4187197..4187982	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
FQT78_RS20195	4188081..4188680	+	600	WP_001315111.1	glucose-1-phosphatase	-
FQT78_RS20200	4188674..4189546	+	873	WP_000920762.1	virulence factor BrkB family protein	-
FQT78_RS20205	4189543..4189980	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FQT78_RS20210	4190025..4190966	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FQT78_RS20215	4191030..4191938	-	909	WP_001162704.1	alpha/beta hydrolase	-
FQT78_RS20220	4192167..4192478	+	312	WP_000897305.1	hypothetical protein	Toxin
FQT78_RS20225	4192479..4192769	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FQT78_RS20230	4193374..4193592	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
FQT78_RS20235	4193811..4194053	+	243	WP_001086388.1	hypothetical protein	-
FQT78_RS20240	4194383..4195312	-	930	WP_000027702.1	formate dehydrogenase accessory protein FdhE	-
FQT78_RS20245	4195309..4195944	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FQT78_RS20250	4195941..4196843	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T138884 WP_000897305.1 NZ_CP043487:4192167-4192478 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T138884 NZ_CP058251:1436818-1437099 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCTGGGCGTATTAAAGAAAGCGA
TGTTACAAGCATTGTTGATTGCCTAAAAGAACAAAAACCACTCCAACAAAAATATTGCGACCATGCCTTGAGTGGCAATC
TTAAAGGGCTGAGAGAGTGTCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT138884 WP_000356397.1 NZ_CP043487:4192479-4192769 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT138884 NZ_CP058251:1436511-1436825 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCATAATTTCTTAAACTCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACGAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACCAAGTTTGTTAAATACGATTGCCTCAAAAGATGACCCAGAA
TACGATGTTTCGTTTGGAGACTTCAATGATTTTCTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7