Detailed information of TA system TA138854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4177806..4178408
• Replicon: chromosome
• Accession: NZ_CP043486
• Organism: Escherichia coli strain SL112

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FQT94_RS20175
• Protein ID: WP_000897305.1
• Coordinates: 4177806..4178117 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FQT94_RS20180
• Protein ID: WP_000356397.1
• Coordinates: 4178118..4178408 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQT94_RS20145	4172836..4173621	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
FQT94_RS20150	4173720..4174319	+	600	WP_001315111.1	glucose-1-phosphatase	-
FQT94_RS20155	4174313..4175185	+	873	WP_000920762.1	virulence factor BrkB family protein	-
FQT94_RS20160	4175182..4175619	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FQT94_RS20165	4175664..4176605	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FQT94_RS20170	4176669..4177577	-	909	WP_001162704.1	alpha/beta hydrolase	-
FQT94_RS20175	4177806..4178117	+	312	WP_000897305.1	hypothetical protein	Toxin
FQT94_RS20180	4178118..4178408	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FQT94_RS20185	4179013..4179231	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
FQT94_RS20190	4179450..4179692	+	243	WP_001086388.1	hypothetical protein	-
FQT94_RS20195	4180022..4180951	-	930	WP_000027702.1	formate dehydrogenase accessory protein FdhE	-
FQT94_RS20200	4180948..4181583	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FQT94_RS20205	4181580..4182482	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T138854 WP_000897305.1 NZ_CP043486:4177806-4178117 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T138854 NZ_CP058236:c2431063-2430746 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT138854 WP_000356397.1 NZ_CP043486:4178118-4178408 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT138854 NZ_CP043486:4178118-4178408 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7