Detailed information of TA system TA138851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3358165..3358964
• Replicon: chromosome
• Accession: NZ_CP043486
• Organism: Escherichia coli strain SL112

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: FQT94_RS16180
• Protein ID: WP_000347267.1
• Coordinates: 3358500..3358964 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: FQT94_RS16175
• Protein ID: WP_001307405.1
• Coordinates: 3358165..3358500 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQT94_RS16160	3353950..3354720	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
FQT94_RS16165	3354736..3356070	-	1335	WP_153986255.1	galactarate/glucarate/glycerate transporter GarP	-
FQT94_RS16170	3356445..3358016	+	1572	WP_001273741.1	galactarate dehydratase	-
FQT94_RS16175	3358165..3358500	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
FQT94_RS16180	3358500..3358964	+	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
FQT94_RS16185	3359019..3359828	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
FQT94_RS16190	3360077..3361357	+	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
FQT94_RS16195	3361380..3361853	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
FQT94_RS16200	3361864..3362643	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
FQT94_RS16205	3362633..3363511	+	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
FQT94_RS16210	3363529..3363963	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3349017..3358964	9947

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T138851 WP_000347267.1 NZ_CP043486:3358500-3358964 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T138851 NZ_CP058236:2278485-2278883 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT138851 WP_001307405.1 NZ_CP043486:3358165-3358500 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT138851 NZ_CP058236:2278257-2278484 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4

Antitoxin

• AlphaFold DB: A0A0E0XVC7