Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2161308..2161810 Replicon chromosome
Accession NZ_CP043486
Organism Escherichia coli strain SL112

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FQT94_RS10525 Protein ID WP_000767829.1
Coordinates 2161308..2161562 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FQT94_RS10530 Protein ID WP_001259255.1
Coordinates 2161559..2161810 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FQT94_RS10495 2156323..2156550 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
FQT94_RS10500 2156564..2157622 - 1059 WP_000492346.1 transport protein YeeE -
FQT94_RS10505 2157801..2159159 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FQT94_RS10510 2159149..2159211 - 63 WP_010723108.1 membrane protein YoeI -
FQT94_RS10515 2159426..2160355 - 930 WP_000803351.1 LysR family transcriptional regulator -
FQT94_RS10520 2160401..2161225 - 825 WP_000754749.1 SDR family oxidoreductase -
FQT94_RS10525 2161308..2161562 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FQT94_RS10530 2161559..2161810 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FQT94_RS10535 2162093..2162143 + 51 WP_001364200.1 his operon leader peptide -
FQT94_RS10540 2162289..2163188 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
FQT94_RS10545 2163194..2164498 + 1305 WP_001349967.1 histidinol dehydrogenase -
FQT94_RS10550 2164495..2165565 + 1071 WP_000108957.1 histidinol-phosphate transaminase -
FQT94_RS10555 2165565..2166632 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T138847 WP_000767829.1 NZ_CP043486:c2161562-2161308 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T138847 NZ_CP043486:c2161562-2161308 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT138847 WP_001259255.1 NZ_CP043486:c2161810-2161559 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT138847 NZ_CP058236:c2229197-2228946 [Mycobacterium tuberculosis]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References