Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 15933..16468 Replicon plasmid pDMEA1
Accession NZ_CP043485
Organism Enterococcus faecium strain DMEA02

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag FZP47_RS12775 Protein ID WP_002314366.1
Coordinates 15933..16205 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag FZP47_RS12780 Protein ID WP_002297422.1
Coordinates 16205..16468 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZP47_RS12745 11263..12540 - 1278 WP_080481984.1 S8 family serine peptidase -
FZP47_RS12750 12570..13298 - 729 WP_002336727.1 hypothetical protein -
FZP47_RS12755 13558..14106 - 549 WP_002335394.1 hypothetical protein -
FZP47_RS12760 14107..14964 - 858 WP_002292680.1 AAA family ATPase -
FZP47_RS12765 15250..15537 - 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
FZP47_RS12770 15527..15856 - 330 WP_002292678.1 AbrB family transcriptional regulator -
FZP47_RS12775 15933..16205 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
FZP47_RS12780 16205..16468 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FZP47_RS12785 16702..17610 + 909 WP_002295743.1 IS982-like element ISEfm1 family transposase -
FZP47_RS12790 17613..18788 - 1176 WP_002336205.1 MFS transporter -
FZP47_RS12795 18791..21040 - 2250 WP_002314387.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ClpL bopD 1..168626 168626
- flank IS/Tn - - 10150..11103 953


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T138827 WP_002314366.1 NZ_CP043485:c16205-15933 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T138827 NZ_CP058236:722448-722855 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT138827 WP_002297422.1 NZ_CP043485:c16468-16205 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT138827 NZ_CP043485:c16468-16205 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References