Detailed information of TA system TA138825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4870013..4870615
• Replicon: chromosome
• Accession: NZ_CP043478
• Organism: Escherichia coli strain ST130

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FZ043_RS24365
• Protein ID: WP_000897305.1
• Coordinates: 4870304..4870615 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FZ043_RS24360
• Protein ID: WP_000356397.1
• Coordinates: 4870013..4870303 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZ043_RS24335	4865939..4866841	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FZ043_RS24340	4866838..4867473	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FZ043_RS24345	4867470..4868399	+	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
FZ043_RS24350	4868729..4868971	-	243	WP_001087409.1	hypothetical protein	-
FZ043_RS24355	4869190..4869408	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
FZ043_RS24360	4870013..4870303	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FZ043_RS24365	4870304..4870615	-	312	WP_000897305.1	hypothetical protein	Toxin
FZ043_RS24370	4870844..4871752	+	909	WP_001162704.1	alpha/beta hydrolase	-
FZ043_RS24375	4871816..4872757	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FZ043_RS24380	4872802..4873239	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FZ043_RS24385	4873236..4874108	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FZ043_RS24390	4874102..4874701	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T138825 WP_000897305.1 NZ_CP043478:c4870615-4870304 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T138825 NZ_CP058236:715171-715572 [Mycobacterium tuberculosis]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT138825 WP_000356397.1 NZ_CP043478:c4870303-4870013 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT138825 NZ_CP058236:714947-715174 [Mycobacterium tuberculosis]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7