Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 959710..960251 Replicon chromosome
Accession NZ_CP043478
Organism Escherichia coli strain ST130

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag FZ043_RS04455 Protein ID WP_000615976.1
Coordinates 959710..959988 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FZ043_RS04460 Protein ID WP_000729704.1
Coordinates 959991..960251 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZ043_RS04440 957292..957870 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
FZ043_RS04445 958076..958843 + 768 WP_000333380.1 class II glutamine amidotransferase -
FZ043_RS04450 958814..959554 - 741 WP_001225679.1 murein L,D-transpeptidase -
FZ043_RS04455 959710..959988 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FZ043_RS04460 959991..960251 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FZ043_RS04465 960437..961210 + 774 WP_000543895.1 C40 family peptidase -
FZ043_RS04470 961267..961623 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
FZ043_RS04475 961616..961894 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
FZ043_RS04480 961999..963711 - 1713 Protein_852 flagellar biosynthesis protein FlhA -
FZ043_RS04485 963683..964468 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 950761..961894 11133


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T138807 WP_000615976.1 NZ_CP043478:c959988-959710 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T138807 NZ_CP058227:c6702-6388 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT138807 WP_000729704.1 NZ_CP043478:c960251-959991 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT138807 NZ_CP058227:c6968-6690 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References