Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 518672..519315 Replicon plasmid unnamed1
Accession NZ_CP043441
Organism Cupriavidus campinensis strain MJ1

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag FZF10_RS21115 Protein ID WP_149137885.1
Coordinates 518672..518971 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag FZF10_RS21120 Protein ID WP_149137886.1
Coordinates 518968..519315 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZF10_RS21095 514095..515812 - 1718 Protein_471 mechanosensitive ion channel -
FZF10_RS21100 515909..516385 - 477 WP_149137883.1 hypothetical protein -
FZF10_RS21105 516530..517231 - 702 WP_144198247.1 3,4-dihydroxy-2-butanone-4-phosphate synthase -
FZF10_RS21110 517605..518495 - 891 WP_149137884.1 helix-turn-helix domain-containing protein -
FZF10_RS21115 518672..518971 + 300 WP_149137885.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FZF10_RS21120 518968..519315 + 348 WP_149137886.1 HigA family addiction module antidote protein Antitoxin
FZF10_RS21125 519458..520771 + 1314 WP_149137887.1 hemolysin family protein -
FZF10_RS21130 521148..522470 + 1323 WP_149137888.1 bifunctional diguanylate cyclase/phosphodiesterase -
FZF10_RS21135 522615..522746 + 132 WP_149137889.1 entericidin A/B family lipoprotein -
FZF10_RS21140 522893..523534 - 642 WP_149137890.1 bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - plc / adeG / acrB / hcp/tssD / vipB/tssC / katA / hsiC1/vipB / hsiB1/vipA / motA / cheA / cheW / cheB / cheY / flhA / flgC / flgG / flgI / fliI / ddhA 1..2786414 2786414


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-85)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11235.81 Da        Isoelectric Point: 10.4170

>T138789 WP_149137885.1 NZ_CP043441:518672-518971 [Cupriavidus campinensis]
MIRTFANKATAAVFCGRYVNTLPRHIQATAFRKLGLLDAAASLGDMQAPPANRLEALRGARRGQWSIQIHNQWRICFRFQ
NGEAWDVDIADYHRQPEPA

Download         Length: 300 bp

>T138789 NZ_CP043441:518672-518971 [Cupriavidus campinensis]
ATGATCCGGACGTTCGCCAACAAAGCCACGGCGGCAGTGTTCTGCGGCCGGTACGTCAACACCCTGCCACGGCACATTCA
GGCCACGGCGTTCAGGAAGCTCGGCCTGCTGGACGCGGCAGCCTCGCTGGGCGACATGCAGGCCCCGCCGGCGAACCGGC
TCGAGGCACTGCGCGGCGCGCGGCGTGGGCAATGGAGCATCCAGATTCATAACCAGTGGCGCATCTGCTTCCGTTTCCAG
AACGGCGAAGCATGGGACGTCGACATCGCGGACTATCACCGACAACCGGAGCCAGCATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 12723.57 Da        Isoelectric Point: 7.2044

>AT138789 WP_149137886.1 NZ_CP043441:518968-519315 [Cupriavidus campinensis]
MTIKREDLPRLDATDVETGEPLAPVHPGEILRQDFMAPHGLSANALAIALRVPAPRINDVVRGRRAISPETALRLARYFG
TTPQFWANLQAGYDLRIAQEKAGEKIDRDIQPLTA

Download         Length: 348 bp

>AT138789 NZ_CP058223:c3862729-3862666 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References