Detailed information of TA system TA138767

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 36951..37681
• Replicon: chromosome
• Accession: NZ_CP043438
• Organism: Enterobacter sp. LU1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: FZF21_RS00175
• Protein ID: WP_023302825.1
• Coordinates: 36951..37265 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FZF21_RS00180
• Protein ID: WP_149121133.1
• Coordinates: 37262..37681 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZF21_RS00150	32617..33702	+	1086	Protein_29	cellulase family glycosylhydrolase	-
FZF21_RS00155	33608..34792	-	1185	WP_047737387.1	multidrug efflux MFS transporter EmrD	-
FZF21_RS00160	34968..35801	-	834	WP_015570117.1	DMT family transporter	-
FZF21_RS00165	35864..36310	-	447	WP_149121134.1	GNAT family N-acetyltransferase	-
FZF21_RS00170	36375..36464	-	90	WP_017383683.1	type I toxin-antitoxin system toxin TisB	-
FZF21_RS00175	36951..37265	+	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
FZF21_RS00180	37262..37681	+	420	WP_149121133.1	helix-turn-helix domain-containing protein	Antitoxin
FZF21_RS00185	37828..37926	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
FZF21_RS00190	38033..39721	+	1689	WP_032668900.1	acetolactate synthase large subunit	-
FZF21_RS00195	39725..40012	+	288	WP_015570113.1	acetolactate synthase small subunit	-
FZF21_RS00200	40138..40731	+	594	WP_003861054.1	transcriptional regulator UhpA	-
FZF21_RS00205	40728..42233	+	1506	WP_126542953.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-137)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T138767 WP_023302825.1 NZ_CP043438:36951-37265 [Enterobacter sp. LU1]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T138767 NZ_CP058222:4132927-4133304 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15547.19 Da
• Isoelectric Point: 5.7670

>AT138767 WP_149121133.1 NZ_CP043438:37262-37681 [Enterobacter sp. LU1]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISRYEANRPEIVALRKEMESFPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT138767 NZ_CP058222:4132506-4132880 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A837FIL2