Detailed information of TA system TA138747

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 100402..100828
• Replicon: plasmid pNTEC2-42405
• Accession: NZ_CP043415
• Organism: Escherichia coli strain EC42405

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FZN31_RS24630
• Protein ID: WP_001312861.1
• Coordinates: 100402..100560 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	100604..100828 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZN31_RS24600	95456..95671	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
FZN31_RS24605	95807..96454	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
FZN31_RS24610	96645..97028	-	384	WP_001063020.1	relaxosome protein TraM	-
FZN31_RS24615	97368..97970	+	603	WP_000243707.1	transglycosylase SLT domain-containing protein	-
FZN31_RS24620	98266..99087	-	822	Protein_99	DUF945 domain-containing protein	-
FZN31_RS24625	99207..99494	-	288	WP_000107542.1	hypothetical protein	-
FZN31_RS24955	99519..99725	-	207	WP_032192990.1	hypothetical protein	-
FZN31_RS24630	100402..100560	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	100604..100828	-	225	NuclAT_0	-	Antitoxin
-	100604..100828	-	225	NuclAT_0	-	Antitoxin
-	100604..100828	-	225	NuclAT_0	-	Antitoxin
-	100604..100828	-	225	NuclAT_0	-	Antitoxin
FZN31_RS24635	100640..100828	+	189	WP_001299721.1	hypothetical protein	-
FZN31_RS24640	100840..101559	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
FZN31_RS24645	101556..101990	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FZN31_RS24650	102045..102833	-	789	Protein_106	hypothetical protein	-
FZN31_RS24655	102916..103938	+	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
FZN31_RS24660	103935..104717	+	783	WP_001300609.1	IS21-like element IS100kyp family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	f17d-G / f17d-C / f17d-D / f17d-A / hlyC / cnf1 / cdtA / cdtB / cdtC / iutA / iucD / iucC / iucB / iucA	1..138121	138121

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T138747 WP_001312861.1 NZ_CP043415:c100560-100402 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T138747 NZ_CP058220:4002626-4003003 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT138747 NZ_CP043415:c100828-100604 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGTGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin