Detailed information of TA system TA138740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 4034818..4035359
• Replicon: chromosome
• Accession: NZ_CP043414
• Organism: Escherichia coli strain EC42405

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: FZN31_RS19225
• Protein ID: WP_057696079.1
• Coordinates: 4035081..4035359 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A7W4KQF3
• Locus tag: FZN31_RS19220
• Protein ID: WP_032183287.1
• Coordinates: 4034818..4035078 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZN31_RS19195	4030635..4031420	-	786	WP_161399933.1	putative lateral flagellar export/assembly protein LafU	-
FZN31_RS19200	4031392..4033104	+	1713	Protein_3761	flagellar biosynthesis protein FlhA	-
FZN31_RS19205	4033209..4033487	+	279	WP_000598759.1	type II toxin-antitoxin system HicA family toxin	-
FZN31_RS19210	4033480..4033836	+	357	WP_001030482.1	type II toxin-antitoxin system HicB family antitoxin	-
FZN31_RS19215	4033859..4034632	-	774	WP_123057343.1	C40 family peptidase	-
FZN31_RS19220	4034818..4035078	+	261	WP_032183287.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
FZN31_RS19225	4035081..4035359	+	279	WP_057696079.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
FZN31_RS19230	4035515..4036255	+	741	WP_001225681.1	murein L,D-transpeptidase	-
FZN31_RS19235	4036226..4036993	-	768	WP_000333380.1	class II glutamine amidotransferase	-
FZN31_RS19240	4037199..4037777	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / rhs/PAAR	4033480..4050074	16594

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10816.55 Da
• Isoelectric Point: 9.9107

>T138740 WP_057696079.1 NZ_CP043414:4035081-4035359 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHNDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T138740 NZ_CP043414:4035081-4035359 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAATGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCACTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTTGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9391.73 Da
• Isoelectric Point: 4.7539

>AT138740 WP_032183287.1 NZ_CP043414:4034818-4035078 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDQLGI

Nucleotide

• Length: 261 bp

>AT138740 NZ_CP058220:c1915170-1914802 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A7W4KQF3