Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2639171..2639733 Replicon chromosome
Accession NZ_CP043414
Organism Escherichia coli strain EC42405

Toxin (Protein)


Gene name higB Uniprot ID A0A2K3TT64
Locus tag FZN31_RS12640 Protein ID WP_032303016.1
Coordinates 2639171..2639449 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag FZN31_RS12645 Protein ID WP_000781370.1
Coordinates 2639449..2639733 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZN31_RS12615 2634758..2635189 - 432 WP_000152305.1 peroxiredoxin OsmC -
FZN31_RS12620 2635533..2635748 + 216 WP_105279170.1 biofilm-dependent modulation protein -
FZN31_RS12625 2635850..2635987 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
FZN31_RS12630 2636144..2637841 + 1698 WP_000433471.1 malate dehydrogenase -
FZN31_RS12635 2637975..2638985 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
FZN31_RS12640 2639171..2639449 + 279 WP_032303016.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FZN31_RS12645 2639449..2639733 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
FZN31_RS12650 2639962..2640615 - 654 WP_000045645.1 formate dehydrogenase-N subunit gamma -
FZN31_RS12655 2640608..2641492 - 885 WP_001240580.1 formate dehydrogenase N subunit beta -
FZN31_RS12660 2641505..2644552 - 3048 WP_015953395.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10535.13 Da        Isoelectric Point: 7.3562

>T138732 WP_032303016.1 NZ_CP043414:2639171-2639449 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQIKRLRHRLAVIDAASCLADLDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T138732 NZ_CP058220:c47349-46924 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCAATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGGTCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCGACCGGCCTGCGGATTCAGTG
CAAAAACACGAATATAATCAGCCGGATGGCTGATTCAAGACATGATAAAAGACTTTACCAGCGCACGTTTAAAGCGTGCA
AGAGATGTTATTATACTAAAACATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT138732 WP_000781370.1 NZ_CP043414:2639449-2639733 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT138732 NZ_CP058220:c47715-47380 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGTTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K3TT64

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References