Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2024544..2025046 Replicon chromosome
Accession NZ_CP043414
Organism Escherichia coli strain EC42405

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FZN31_RS09515 Protein ID WP_000767829.1
Coordinates 2024792..2025046 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FZN31_RS09510 Protein ID WP_001259255.1
Coordinates 2024544..2024795 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZN31_RS09485 2019722..2020789 - 1068 WP_000080067.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FZN31_RS09490 2020789..2021859 - 1071 WP_001099221.1 histidinol-phosphate transaminase -
FZN31_RS09495 2021856..2023160 - 1305 WP_001295420.1 histidinol dehydrogenase -
FZN31_RS09500 2023166..2024065 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FZN31_RS09505 2024211..2024261 - 51 WP_001364200.1 his operon leader peptide -
FZN31_RS09510 2024544..2024795 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FZN31_RS09515 2024792..2025046 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FZN31_RS09520 2025129..2025953 + 825 WP_000754737.1 SDR family oxidoreductase -
FZN31_RS09525 2025999..2026928 + 930 WP_000803351.1 LysR family transcriptional regulator -
FZN31_RS09530 2027143..2027205 + 63 WP_010723108.1 membrane protein YoeI -
FZN31_RS09535 2027195..2028553 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FZN31_RS09540 2028732..2029790 + 1059 WP_000492325.1 transport protein YeeE -
FZN31_RS09545 2029804..2030031 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T138721 WP_000767829.1 NZ_CP043414:2024792-2025046 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T138721 NZ_CP058217:2814433-2814540 [Escherichia coli Nissle 1917]
ATGACGCTCGCGCAGTTTGCCGTGATTTTCTGGCACGACCTGGCAGCACCGATCCTTGCGGGAATTATTACCGCAGCGAT
TGTTGGCTGGTGGCGTAGCCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT138721 WP_001259255.1 NZ_CP043414:2024544-2024795 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT138721 NZ_CP043414:2024544-2024795 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References