Detailed information of TA system TA138720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1215850..1216577
• Replicon: chromosome
• Accession: NZ_CP043414
• Organism: Escherichia coli strain EC42405

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6N9MSG8
• Locus tag: FZN31_RS05795
• Protein ID: WP_032184987.1
• Coordinates: 1215850..1216161 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FZN31_RS05800
• Protein ID: WP_000126291.1
• Coordinates: 1216158..1216577 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZN31_RS05765	1210991..1212700	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
FZN31_RS05770	1212710..1213252	+	543	WP_000493782.1	formate hydrogenlyase subunit HycF	-
FZN31_RS05775	1213252..1214019	+	768	WP_000067390.1	formate hydrogenlyase subunit HycG	-
FZN31_RS05780	1214016..1214426	+	411	WP_001291919.1	formate hydrogenlyase assembly protein HycH	-
FZN31_RS05785	1214419..1214889	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
FZN31_RS05790	1214932..1215687	+	756	WP_032184988.1	hypothetical protein	-
FZN31_RS05795	1215850..1216161	+	312	WP_032184987.1	type II toxin-antitoxin system HigB family toxin	Toxin
FZN31_RS05800	1216158..1216577	+	420	WP_000126291.1	helix-turn-helix domain-containing protein	Antitoxin
FZN31_RS05805	1216691..1218115	-	1425	WP_032185108.1	6-phospho-beta-glucosidase AscB	-
FZN31_RS05810	1218124..1219581	-	1458	WP_001107840.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
FZN31_RS05815	1219841..1220851	+	1011	WP_032185107.1	DNA-binding transcriptional regulator AscG	-
FZN31_RS05820	1221000..1221527	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12451.14 Da
• Isoelectric Point: 9.7249

>T138720 WP_032184987.1 NZ_CP043414:1215850-1216161 [Escherichia coli]
MHIISKAPFEESARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENRE

Nucleotide

• Length: 312 bp

>T138720 NZ_CP058217:2576190-2576468 [Escherichia coli Nissle 1917]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15439.46 Da
• Isoelectric Point: 4.5628

>AT138720 WP_000126291.1 NZ_CP043414:1216158-1216577 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNHEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT138720 NZ_CP058217:2576468-2576752 [Escherichia coli Nissle 1917]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: A0A6N9MSG8