Detailed information of TA system TA138717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 758743..759470
• Replicon: chromosome
• Accession: NZ_CP043414
• Organism: Escherichia coli strain EC42405

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FZN31_RS03645
• Protein ID: WP_105287707.1
• Coordinates: 758743..759057 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FZN31_RS03650
• Protein ID: WP_000560266.1
• Coordinates: 759054..759470 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZN31_RS03625	754900..755886	-	987	WP_088541800.1	Gfo/Idh/MocA family oxidoreductase	-
FZN31_RS03630	755965..756657	-	693	WP_032185166.1	vancomycin high temperature exclusion protein	-
FZN31_RS03635	756734..757237	-	504	WP_001295542.1	M48 family metallopeptidase	-
FZN31_RS03640	757322..758458	+	1137	WP_000018675.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
FZN31_RS03645	758743..759057	+	315	WP_105287707.1	type II toxin-antitoxin system toxin HigB	Toxin
FZN31_RS03650	759054..759470	+	417	WP_000560266.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
FZN31_RS03655	759515..761533	-	2019	WP_105287706.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
FZN31_RS03660	761856..764207	-	2352	WP_105287705.1	alpha-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12121.14 Da
• Isoelectric Point: 10.0409

>T138717 WP_105287707.1 NZ_CP043414:758743-759057 [Escherichia coli]
MHMITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T138717 NZ_CP043414:758743-759057 [Escherichia coli]
ATGCACATGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT138717 WP_000560266.1 NZ_CP043414:759054-759470 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT138717 NZ_CP058217:c1918260-1917892 [Escherichia coli Nissle 1917]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA