Detailed information of TA system TA138712

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25882..26151
• Replicon: plasmid pNMBU-W13E19_02
• Accession: NZ_CP043408
• Organism: Escherichia coli strain NMBU-W13E19

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FZN32_RS25640
• Protein ID: WP_001312861.1
• Coordinates: 25993..26151 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	25882..25947 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZN32_RS25605	21171..21364	+	194	Protein_28	hypothetical protein	-
FZN32_RS25610	21592..22119	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
FZN32_RS25615	22177..22410	+	234	WP_000006003.1	DUF905 family protein	-
FZN32_RS25620	22471..24494	+	2024	Protein_31	ParB/RepB/Spo0J family partition protein	-
FZN32_RS25625	24563..24997	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FZN32_RS25630	24994..25713	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
FZN32_RS25635	25725..25913	-	189	WP_001299721.1	hypothetical protein	-
-	25725..25949	+	225	NuclAT_0	-	-
-	25725..25949	+	225	NuclAT_0	-	-
-	25725..25949	+	225	NuclAT_0	-	-
-	25725..25949	+	225	NuclAT_0	-	-
-	25882..25947	+	66	-	-	Antitoxin
FZN32_RS25640	25993..26151	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
FZN32_RS25645	26647..26840	+	194	Protein_36	hypothetical protein	-
FZN32_RS25650	27066..27362	+	297	WP_001272251.1	hypothetical protein	-
FZN32_RS25655	27473..28294	+	822	WP_001234445.1	DUF945 domain-containing protein	-
FZN32_RS25660	28591..29193	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
FZN32_RS25665	29516..29899	+	384	WP_001354030.1	relaxosome protein TraM	-
FZN32_RS25670	30093..30764	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
FZN32_RS25675	30901..31128	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-15	-	1..67044	67044

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T138712 WP_001312861.1 NZ_CP043408:25993-26151 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T138712 NZ_CP058217:c842693-842316 [Escherichia coli Nissle 1917]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT138712 NZ_CP043408:25882-25947 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin