Detailed information of TA system TA138701

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 49170..49409
• Replicon: plasmid pNMBU-W13E19_01
• Accession: NZ_CP043407
• Organism: Escherichia coli strain NMBU-W13E19

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: FZN32_RS25015
• Protein ID: WP_023144756.1
• Coordinates: 49170..49304 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	49349..49409 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZN32_RS24980	44756..44896	-	141	Protein_58	DUF262 domain-containing protein	-
FZN32_RS24985	45181..45582	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
FZN32_RS24990	45515..45772	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
FZN32_RS24995	45865..46518	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
FZN32_RS25000	47458..48315	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
FZN32_RS25005	48308..48382	-	75	WP_031943482.1	RepA leader peptide Tap	-
FZN32_RS25010	48619..48873	-	255	WP_000083850.1	replication regulatory protein RepA	-
FZN32_RS25015	49170..49304	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	49349..49409	+	61	NuclAT_1	-	Antitoxin
-	49349..49409	+	61	NuclAT_1	-	Antitoxin
-	49349..49409	+	61	NuclAT_1	-	Antitoxin
-	49349..49409	+	61	NuclAT_1	-	Antitoxin
FZN32_RS25020	49376..49662	-	287	Protein_66	DUF2726 domain-containing protein	-
FZN32_RS25025	50175..50387	-	213	WP_013023861.1	hypothetical protein	-
FZN32_RS25030	50518..51078	-	561	WP_000139341.1	fertility inhibition protein FinO	-
FZN32_RS25035	51133..51879	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / dfrA14 / mph(A)	-	1..122112	122112
	-	flank	IS/Tn	-	-	44003..44380	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T138701 WP_023144756.1 NZ_CP043407:c49304-49170 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T138701 NZ_CP043407:c49304-49170 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT138701 NZ_CP043407:49349-49409 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin