Detailed information of TA system TA138685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2164907..2165557
• Replicon: chromosome
• Accession: NZ_CP043406
• Organism: Escherichia coli strain NMBU-W13E19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FZN32_RS10785
• Protein ID: WP_000263532.1
• Coordinates: 2164907..2165248 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: FZN32_RS10790
• Protein ID: WP_000212552.1
• Coordinates: 2165258..2165557 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZN32_RS10760	2159942..2161042	+	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-
FZN32_RS10765	2161042..2161341	+	300	WP_000867281.1	rod-binding protein	-
FZN32_RS10770	2161517..2162893	+	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
FZN32_RS10775	2162908..2163837	+	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
FZN32_RS10780	2163854..2164831	+	978	WP_001195938.1	hypothetical protein	-
FZN32_RS10785	2164907..2165248	+	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FZN32_RS10790	2165258..2165557	+	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
FZN32_RS10795	2165615..2166457	-	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
FZN32_RS10800	2166943..2167857	+	915	WP_000949083.1	lateral flagellin LafA	-
FZN32_RS10805	2168062..2169378	+	1317	WP_000609678.1	flagellar filament capping protein FliD	-
FZN32_RS10810	2169401..2169793	+	393	WP_000285322.1	flagellar export chaperone FliS	-
FZN32_RS10815	2169798..2170109	+	312	WP_000415004.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T138685 WP_000263532.1 NZ_CP043406:2164907-2165248 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T138685 NZ_CP043406:2164907-2165248 [Escherichia coli]
ATGTGGGACGTCGAGACAACGGAGACTTTCGACAACTGGTTTGATGCCCAGACTGTTGCGCTGAAAGAAGATTTACTTGC
TGCAATGCTGATTCTTGCTGAGTATGGCCCACAGTTGGGCAGACCCTTTGCGGATACGGTTAATGATTCACAGTTTTCAA
ATATGAAAGAATTACGCGTCCAACATCAGGGAAACCCGGTACGTGCTTTTTTTGCTTTCGATCCTGCGCGCCGTGGCATT
GTATTATGTGCGGGTGATAAAACGGGGTTAAATGAGAAAAAGTTTTATCGCGATATGATCAAGCTCGCTGATTCTGAATA
CCGTAAGCATCTGAAAAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT138685 WP_000212552.1 NZ_CP043406:2165258-2165557 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT138685 NZ_CP058209:1063969-1064027 [Escherichia marmotae]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7