Detailed information of TA system TA138669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 110536..111038
• Replicon: chromosome
• Accession: NZ_CP043406
• Organism: Escherichia coli strain NMBU-W13E19

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: FZN32_RS00555
• Protein ID: WP_000767819.1
• Coordinates: 110784..111038 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: FZN32_RS00550
• Protein ID: WP_001259253.1
• Coordinates: 110536..110787 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZN32_RS00525	105714..106781	-	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
FZN32_RS00530	106781..107851	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
FZN32_RS00535	107848..109152	-	1305	WP_001296211.1	histidinol dehydrogenase	-
FZN32_RS00540	109158..110057	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
FZN32_RS00545	110203..110253	-	51	WP_001364200.1	his operon leader peptide	-
FZN32_RS00550	110536..110787	+	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
FZN32_RS00555	110784..111038	+	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
FZN32_RS00560	111121..111945	+	825	WP_000754737.1	SDR family oxidoreductase	-
FZN32_RS00565	111991..112920	+	930	WP_000803366.1	LysR family transcriptional regulator	-
FZN32_RS00570	113135..113197	+	63	WP_010723108.1	membrane protein YoeI	-
FZN32_RS00575	113187..114545	+	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
FZN32_RS00580	114762..115220	+	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	114762..115220	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T138669 WP_000767819.1 NZ_CP043406:110784-111038 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T138669 NZ_CP058207:c610036-609695 [Escherichia marmotae]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGCCGCTACCCGAATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCGA
CCGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCTCCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGTACGTAAACAAAAGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT138669 WP_001259253.1 NZ_CP043406:110536-110787 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT138669 NZ_CP058207:610031-610107 [Escherichia marmotae]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3