Detailed information of TA system TA138645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1140746..1141395
• Replicon: chromosome
• Accession: NZ_CP043398
• Organism: Leclercia adecarboxylata strain G426

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FY047_RS05290
• Protein ID: WP_103791861.1
• Coordinates: 1141033..1141395 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FY047_RS05285
• Protein ID: WP_059308573.1
• Coordinates: 1140746..1141045 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY047_RS05275	1137142..1139115	+	1974	WP_032616918.1	PhoX family phosphatase	-
FY047_RS05280	1139219..1140604	+	1386	WP_077226217.1	phenylalanine transporter	-
FY047_RS05285	1140746..1141045	-	300	WP_059308573.1	helix-turn-helix transcriptional regulator	Antitoxin
FY047_RS05290	1141033..1141395	-	363	WP_103791861.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY047_RS05295	1141672..1142913	+	1242	WP_032616942.1	MFS transporter	-
FY047_RS05300	1142925..1143950	+	1026	WP_103791862.1	sugar phosphate isomerase/epimerase	-
FY047_RS05305	1143943..1145085	+	1143	WP_077226215.1	Gfo/Idh/MocA family oxidoreductase	-
FY047_RS05310	1145161..1146141	+	981	WP_103791863.1	LacI family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13887.78 Da
• Isoelectric Point: 6.0839

>T138645 WP_103791861.1 NZ_CP043398:c1141395-1141033 [Leclercia adecarboxylata]
MWEVETTDTFDKWFNHQTQALKEDVLAIMHILSEYGPQLGRPWVDTVKNSHYANMKELRIQHAGDPVRAFFAFDPGCKGI
ILCAGNKTGKNEKRFYKDMITIADAEFSDYLASKEAEWLR

Nucleotide

• Length: 363 bp

>T138645 NZ_CP058187:c4710937-4710566 [Enterobacter hormaechei]
ATGGAGCACATGGCTGTGTTTGACACCAATATCCTCATCGATCTTTTTAATAACCACGTTGAGGCTGCGGATGCGATCGA
TCGCACAGCTTCGCATCGTGCCATTAGCCTCATCACATGGATGGAAGTAATGGTGGGCGCTCGCAGGCACGGTCAAGAAG
CAAAAACGGCAGCCGTGATGGAGGCCTTTGAAATTATTGATATAACAAGGGAAATTGCTGAAAGAAGCGTATTACTTCGC
GAACAGCACGGGATGAAGCTTCCTGACGCCATTATTCTGGCTACTGCTCAGAGCAGAAGCTGTCCGTTAATTTCGCGCAA
TACGAAAGACTTTTCGGGTATTGCTGGGGTCGTTTCACCTTACCACCTGTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10972.68 Da
• Isoelectric Point: 5.8526

>AT138645 WP_059308573.1 NZ_CP043398:c1141045-1140746 [Leclercia adecarboxylata]
MATLKELMAQQSQESQERIAAKVEDLRIIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPDNDPRLSTLKRYVTALG
GEVSIDVILPTGKRVAFHF

Nucleotide

• Length: 300 bp

>AT138645 NZ_CP058187:c4711182-4710940 [Enterobacter hormaechei]
ATGAGTCTTTTGGCGGACCTTACTATGGGCAGAATACTGATCGATTTGTCTGATGACGTGATTCAACGACTGGATAACCT
GAAGCAGTTGCGCAACCGGCCGCGCGCCGAACTGCTGCGGGAAGCCATAGAACTGTATCTCGACCAGCAGAATTCATCGG
TTATCCGCGAAGCGCTTGGCCTGTGGGGAAATCAGCAGGAAGATGGGCTTGAGTACGAACGCAAGCTGCGTGAGGAGTGG
TAA