Detailed information of TA system TA138632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1212956..1213605
• Replicon: chromosome
• Accession: NZ_CP043397
• Organism: Leclercia adecarboxylata strain L21

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FY044_RS05825
• Protein ID: WP_077226216.1
• Coordinates: 1213243..1213605 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FY044_RS05820
• Protein ID: WP_059308573.1
• Coordinates: 1212956..1213255 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY044_RS05810	1209352..1211325	+	1974	WP_032616918.1	PhoX family phosphatase	-
FY044_RS05815	1211429..1212814	+	1386	WP_077226217.1	phenylalanine transporter	-
FY044_RS05820	1212956..1213255	-	300	WP_059308573.1	helix-turn-helix transcriptional regulator	Antitoxin
FY044_RS05825	1213243..1213605	-	363	WP_077226216.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY044_RS05830	1213882..1215123	+	1242	WP_077226245.1	MFS transporter	-
FY044_RS05835	1215135..1216160	+	1026	WP_059308572.1	sugar phosphate isomerase/epimerase	-
FY044_RS05840	1216153..1217295	+	1143	WP_077226215.1	Gfo/Idh/MocA family oxidoreductase	-
FY044_RS05845	1217371..1218351	+	981	WP_032616924.1	LacI family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14014.03 Da
• Isoelectric Point: 7.3565

>T138632 WP_077226216.1 NZ_CP043397:c1213605-1213243 [Leclercia adecarboxylata]
MWEVETTDTFDKWFNHQTQALKEDVLAIMHILSEYGPQLGRPWVDTVKNSHYANMKELRIQHAGDPVRAFFAFDPVRKAI
ILCAGNKTGKNEKRFYKDMITMADAEFSDYLASKEAKWLR

Nucleotide

• Length: 363 bp

>T138632 NZ_CP043397:c1213605-1213243 [Leclercia adecarboxylata]
ATGTGGGAAGTCGAAACAACGGATACGTTTGACAAGTGGTTTAACCACCAGACACAAGCCTTGAAAGAAGATGTCCTGGC
TATAATGCATATCCTCTCAGAGTACGGTCCACAGCTGGGCCGTCCCTGGGTCGATACAGTTAAAAACTCACATTACGCGA
ATATGAAAGAGCTACGCATTCAGCATGCAGGGGATCCGGTTCGTGCTTTTTTTGCCTTCGATCCTGTTCGTAAAGCCATA
ATTTTATGTGCTGGAAATAAGACGGGAAAAAATGAAAAGCGATTCTATAAAGATATGATCACCATGGCTGATGCTGAATT
CAGCGATTATCTGGCGAGTAAGGAGGCCAAATGGCTACGTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10972.68 Da
• Isoelectric Point: 5.8526

>AT138632 WP_059308573.1 NZ_CP043397:c1213255-1212956 [Leclercia adecarboxylata]
MATLKELMAQQSQESQERIAAKVEDLRIIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPDNDPRLSTLKRYVTALG
GEVSIDVILPTGKRVAFHF

Nucleotide

• Length: 300 bp

>AT138632 NZ_CP058180:2083479-2083781 [Klebsiella grimontii]
ATGGGCAGAACGCTGGAACAGCTGATGGCGGATGAAAAACCGGAAGTCGTGGCTGACGCCCGGGCTATGGCGGCGGACAT
CCTGCTCAACATTCACCTTGCAGAACTGCGTGAGAAAGTACAGAAAACGCAGGTCGAAATGGCGCAGGCGCTGGGCATCA
GGCAACCTACCGTTGCAGGCATGGAAAAACCAGGCCGTGACCTGAAGCTTTCCACGCTTAAACGCTACGTTGAAGCCACC
GGCGGCAAGCTACGTCTTGATGTTGAGTTGCCAGATGGTTCTCACTACGGATTTGAACTGTAA