Detailed information of TA system TA138510

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 153623..153876
• Replicon: plasmid pKPC2_020120
• Accession: NZ_CP043358
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020120

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: CLQ78_RS27855
• Protein ID: WP_001312851.1
• Coordinates: 153727..153876 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	153623..153682 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ78_RS27815 (149283)	149283..149348	-	66	Protein_193	helix-turn-helix domain-containing protein	-
CLQ78_RS27820 (149401)	149401..150105	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
CLQ78_RS27825 (150130)	150130..150330	+	201	WP_072354025.1	hypothetical protein	-
CLQ78_RS27830 (150350)	150350..151096	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
CLQ78_RS27835 (151151)	151151..151711	+	561	WP_000139328.1	fertility inhibition protein FinO	-
CLQ78_RS27840 (151843)	151843..152043	+	201	WP_015059022.1	hypothetical protein	-
CLQ78_RS27845 (152429)	152429..153028	+	600	WP_032083981.1	PIN domain-containing protein	-
CLQ78_RS27850 (153090)	153090..153422	+	333	WP_152916585.1	hypothetical protein	-
- (153623)	153623..153682	-	60	NuclAT_1	-	Antitoxin
- (153623)	153623..153682	-	60	NuclAT_1	-	Antitoxin
- (153623)	153623..153682	-	60	NuclAT_1	-	Antitoxin
- (153623)	153623..153682	-	60	NuclAT_1	-	Antitoxin
CLQ78_RS27855 (153727)	153727..153876	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
CLQ78_RS27860 (154160)	154160..154408	+	249	WP_000083837.1	replication regulatory protein RepA	-
CLQ78_RS29335 (154523)	154523..154707	+	185	Protein_203	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB	-	1..154719	154719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T138510 WP_001312851.1 NZ_CP043358:153727-153876 [Klebsiella pneumoniae subsp. pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T138510 NZ_CP058128:c2795869-2795766 [Enterobacter hormaechei]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 60 bp

>AT138510 NZ_CP043358:c153682-153623 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin