Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 153623..153876 Replicon plasmid pKPC2_020120
Accession NZ_CP043358
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020120

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag CLQ78_RS27855 Protein ID WP_001312851.1
Coordinates 153727..153876 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name sok
Locus tag -
Coordinates 153623..153682 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ78_RS27815 (149283) 149283..149348 - 66 Protein_193 helix-turn-helix domain-containing protein -
CLQ78_RS27820 (149401) 149401..150105 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
CLQ78_RS27825 (150130) 150130..150330 + 201 WP_072354025.1 hypothetical protein -
CLQ78_RS27830 (150350) 150350..151096 + 747 WP_000205770.1 conjugal transfer pilus acetylase TraX -
CLQ78_RS27835 (151151) 151151..151711 + 561 WP_000139328.1 fertility inhibition protein FinO -
CLQ78_RS27840 (151843) 151843..152043 + 201 WP_015059022.1 hypothetical protein -
CLQ78_RS27845 (152429) 152429..153028 + 600 WP_032083981.1 PIN domain-containing protein -
CLQ78_RS27850 (153090) 153090..153422 + 333 WP_152916585.1 hypothetical protein -
- (153623) 153623..153682 - 60 NuclAT_1 - Antitoxin
- (153623) 153623..153682 - 60 NuclAT_1 - Antitoxin
- (153623) 153623..153682 - 60 NuclAT_1 - Antitoxin
- (153623) 153623..153682 - 60 NuclAT_1 - Antitoxin
CLQ78_RS27855 (153727) 153727..153876 + 150 WP_001312851.1 Hok/Gef family protein Toxin
CLQ78_RS27860 (154160) 154160..154408 + 249 WP_000083837.1 replication regulatory protein RepA -
CLQ78_RS29335 (154523) 154523..154707 + 185 Protein_203 protein CopA/IncA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB - 1..154719 154719


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T138510 WP_001312851.1 NZ_CP043358:153727-153876 [Klebsiella pneumoniae subsp. pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T138510 NZ_CP058128:c2795869-2795766 [Enterobacter hormaechei]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 60 bp

>AT138510 NZ_CP043358:c153682-153623 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C8K5

Antitoxin

Download structure file

References