Detailed information of TA system TA138482

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 9657..9910
• Replicon: plasmid pKPC2_020115
• Accession: NZ_CP043354
• Organism: Klebsiella pneumoniae strain WCHKP020115

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: C6M09_RS26810
• Protein ID: WP_001312851.1
• Coordinates: 9761..9910 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	9657..9716 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6M09_RS26770 (4755)	4755..5309	+	555	WP_063840321.1	fluoroquinolone-acetylating aminoglycoside 6'-N-acetyltransferase AAC(6')-Ib-cr5	-
C6M09_RS26775 (5435)	5435..6139	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
C6M09_RS26780 (6164)	6164..6364	+	201	WP_072354025.1	hypothetical protein	-
C6M09_RS26785 (6384)	6384..7130	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
C6M09_RS26790 (7185)	7185..7745	+	561	WP_000139328.1	fertility inhibition protein FinO	-
C6M09_RS26795 (7877)	7877..8077	+	201	WP_015059022.1	hypothetical protein	-
C6M09_RS26800 (8463)	8463..9062	+	600	WP_032083981.1	PIN domain-containing protein	-
C6M09_RS26805 (9124)	9124..9456	+	333	WP_152916585.1	hypothetical protein	-
- (9657)	9657..9716	-	60	NuclAT_0	-	Antitoxin
- (9657)	9657..9716	-	60	NuclAT_0	-	Antitoxin
- (9657)	9657..9716	-	60	NuclAT_0	-	Antitoxin
- (9657)	9657..9716	-	60	NuclAT_0	-	Antitoxin
C6M09_RS26810 (9761)	9761..9910	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
C6M09_RS26815 (10194)	10194..10442	+	249	WP_000083837.1	replication regulatory protein RepA	-
C6M09_RS28095 (10557)	10557..10735	+	179	Protein_13	protein CopA/IncA	-
C6M09_RS26825 (10754)	10754..11611	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
C6M09_RS26830 (12550)	12550..13203	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
C6M09_RS26835 (13296)	13296..13553	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
C6M09_RS26840 (13486)	13486..13887	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
C6M09_RS26845 (14136)	14136..14551	+	416	Protein_18	IS1-like element IS1B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aac(6')-Ib-cr / blaCTX-M-65 / catA2 / blaKPC-2 / blaTEM-1C / tet(A)	-	1..99284	99284
	-	flank	IS/Tn	aac(6')-Ib-cr	-	4710..6139	1429

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T138482 WP_001312851.1 NZ_CP043354:9761-9910 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T138482 NZ_CP058058:2252-2533 [Escherichia fergusonii]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT138482 NZ_CP043354:c9716-9657 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin