Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 355745..356391 Replicon chromosome
Accession NZ_CP043353
Organism Klebsiella pneumoniae strain WCHKP020115

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag C6M09_RS01655 Protein ID WP_002920560.1
Coordinates 355745..356092 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag C6M09_RS01660 Protein ID WP_002920557.1
Coordinates 356092..356391 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M09_RS01645 (C6M09_01645) 351671..353104 + 1434 WP_002920564.1 glycogen synthase GlgA -
C6M09_RS01650 (C6M09_01650) 353122..355569 + 2448 WP_002920561.1 glycogen phosphorylase -
C6M09_RS01655 (C6M09_01655) 355745..356092 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6M09_RS01660 (C6M09_01660) 356092..356391 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
C6M09_RS01665 (C6M09_01665) 356454..357962 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
C6M09_RS01670 (C6M09_01670) 358167..358496 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
C6M09_RS01675 (C6M09_01675) 358547..359377 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
C6M09_RS01680 (C6M09_01680) 359427..360185 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T138465 WP_002920560.1 NZ_CP043353:355745-356092 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T138465 NZ_CP058057:393457-393747 [Escherichia fergusonii]
ATGAACTATGAACTGGCTTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGGGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTACGGGATTTGCCGGACTGCTACAAAATTA
AGCTTCGCACACTGGGATATCGCCGGGTCTATCAGGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT138465 WP_002920557.1 NZ_CP043353:356092-356391 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT138465 NZ_CP058057:393225-393467 [Escherichia fergusonii]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCAGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAGGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References