Detailed information of TA system TA138439

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37144..37413
• Replicon: plasmid pKPC2_020039
• Accession: NZ_CP043345
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020039

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: CVC44_RS26685
• Protein ID: WP_001372321.1
• Coordinates: 37288..37413 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	37144..37209 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CVC44_RS26655	32854..33381	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
CVC44_RS26660	33439..33672	+	234	WP_000006003.1	DUF905 family protein	-
CVC44_RS26665	33733..35756	+	2024	Protein_45	ParB/RepB/Spo0J family partition protein	-
CVC44_RS26670	35825..36259	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CVC44_RS26675	36256..36975	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	36987..37211	+	225	NuclAT_0	-	-
-	36987..37211	+	225	NuclAT_0	-	-
-	36987..37211	+	225	NuclAT_0	-	-
-	36987..37211	+	225	NuclAT_0	-	-
-	37144..37209	+	66	-	-	Antitoxin
CVC44_RS26680	37197..37346	+	150	Protein_48	plasmid maintenance protein Mok	-
CVC44_RS26685	37288..37413	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
CVC44_RS26690	37732..38028	-	297	Protein_50	hypothetical protein	-
CVC44_RS26695	38379..39083	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
CVC44_RS26700	39146..39541	+	396	Protein_52	ATP-dependent endonuclease	-
CVC44_RS26705	39526..40548	+	1023	WP_000361404.1	helicase UvrD	-
CVC44_RS26710	40804..41501	+	698	WP_223174630.1	IS1 family transposase	-
CVC44_RS26715	41530..41802	+	273	Protein_55	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / blaSHV-12	-	1..128614	128614

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T138439 WP_001372321.1 NZ_CP043345:37288-37413 [Klebsiella pneumoniae subsp. pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T138439 NZ_CP057864:3577635-3577853 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT138439 NZ_CP043345:37144-37209 [Klebsiella pneumoniae subsp. pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin