Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 330133..330719 Replicon chromosome
Accession NZ_CP043344
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020039

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag CVC44_RS01530 Protein ID WP_002920800.1
Coordinates 330351..330719 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag CVC44_RS01525 Protein ID WP_002920802.1
Coordinates 330133..330354 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CVC44_RS01505 (CVC44_01515) 326290..327216 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
CVC44_RS01510 (CVC44_01520) 327213..328490 + 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
CVC44_RS01515 (CVC44_01525) 328487..329254 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
CVC44_RS01520 (CVC44_01530) 329256..329969 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
CVC44_RS01525 (CVC44_01535) 330133..330354 + 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CVC44_RS01530 (CVC44_01540) 330351..330719 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CVC44_RS01535 (CVC44_01545) 330992..332308 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
CVC44_RS01540 (CVC44_01550) 332415..333302 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
CVC44_RS01545 (CVC44_01555) 333299..334144 + 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
CVC44_RS01550 (CVC44_01560) 334146..335216 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 327213..335953 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T138421 WP_002920800.1 NZ_CP043344:330351-330719 [Klebsiella pneumoniae subsp. pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T138421 NZ_CP057722:1841983-1842085 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT138421 WP_002920802.1 NZ_CP043344:330133-330354 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT138421 NZ_CP057722:c1842092-1841947 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References